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Literature summary for 1.1.1.37 extracted from

  • Nava, G.; Laclette, J.P.; Bobes, R.; Carrero, J.C.; Reyes-Vivas, H.; Enriquez-Flores, S.; Mendoza-Hernandez, G.; Plancarte, A.
    Cloning, sequencing and functional expression of cytosolic malate dehydrogenase from Taenia solium: purification and characterization of the recombinant enzyme (2011), Exp. Parasitol., 128, 217-224.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cMDH, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, expression of N-terminal His-tagged cMDH in Escherichia coli strain BL21 (DE3) pLysS Taenia solium
expression in Escherichia coli Taenia solium

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: praziquantel and albendazole Taenia solium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
NAD+ pH 8.0, temperature not specified in the publication Taenia solium
0.042
-
NAD+ pH 9.6, 22°C, recombinant enzyme Taenia solium
0.044
-
oxaloacetate pH 8.0, temperature not specified in the publication Taenia solium
0.044
-
oxaloacetate pH 8.0, 22°C, recombinant enzyme Taenia solium
0.1
-
(S)-malate pH 8.0, temperature not specified in the publication Taenia solium
0.1
-
(S)-malate pH 9.6, 22°C, recombinant enzyme Taenia solium
0.167
-
NADH pH 8.0, temperature not specified in the publication Taenia solium
0.167
-
NADH pH 8.0, 22°C, recombinant enzyme Taenia solium

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Taenia solium 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE Taenia solium
36000
-
x * 36517, calculated, x * 36000, SDS-PAGE, recombinant protein Taenia solium
36517
-
x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE Taenia solium
36517
-
x * 36517, calculated, x * 36000, SDS-PAGE, recombinant protein Taenia solium
37340
-
x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE Taenia solium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Taenia solium
-
oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+ Taenia solium
-
(S)-malate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Taenia solium F1C7I4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminal His-tagged cMDH from Escherichia coli strain BL21(DE3) pLysS inclusion bodies by affinity chromatography to over 95% purity Taenia solium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
409
-
pH 8.0, temperature not specified in the publication Taenia solium
409
-
purified recombinant enzyme, oxaloacetate reduction, pH 8.0, 22°C Taenia solium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Taenia solium oxaloacetate + NADH + H+
-
r
additional information no substrate: lactate Taenia solium ?
-
?
oxaloacetate + NADH + H+
-
Taenia solium (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
? x * 36517, cMDH, sequence calculation, x * 37340, C-terminally His-tagged cMDH, sequence calculation, x * 36000, His-tagged cMDH, SDS-PAGE Taenia solium
? x * 36517, calculated, x * 36000, SDS-PAGE, recombinant protein Taenia solium

Synonyms

Synonyms Comment Organism
cMDH
-
Taenia solium
cytosolic malate dehydrogenase
-
Taenia solium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at room temperature Taenia solium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5 40 gradual decrease in activity above 40°C Taenia solium
5 60 purified His-tagged cMDH, high stability within this range Taenia solium
20
-
stable for at least 30 min Taenia solium
37
-
stable for at least 30 min Taenia solium
50.5
-
Tm, melting temperature Taenia solium
78.5
-
purified His-tagged cMDH, high stability within this range Taenia solium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
47
-
(S)-malate pH 9.6, 22°C, recombinant enzyme Taenia solium
47.4
-
(S)-malate pH 8.0, temperature not specified in the publication Taenia solium
385
-
NAD+ pH 9.6, 22°C, recombinant enzyme Taenia solium
385.6
-
NAD+ pH 8.0, temperature not specified in the publication Taenia solium
665
-
oxaloacetate pH 8.0, 22°C, recombinant enzyme Taenia solium
665.3
-
oxaloacetate pH 8.0, temperature not specified in the publication Taenia solium
962
-
NADH pH 8.0, 22°C, recombinant enzyme Taenia solium
962.7
-
NADH pH 8.0, temperature not specified in the publication Taenia solium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
oxaloacetate reduction Taenia solium
8
-
-
Taenia solium
9.6
-
malate oxidation Taenia solium

pH Stability

pH Stability pH Stability Maximum Comment Organism
7 8.5
-
Taenia solium

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine adenine dinucleotide about 30% of the activity with NADH Taenia solium
additional information no activity with NADPH Taenia solium
NAD+
-
Taenia solium
NADH
-
Taenia solium
NADH specific for NADH Taenia solium

pI Value

Organism Comment pI Value Maximum pI Value
Taenia solium isoelectrofocusing
-
8.1
Taenia solium calculated and isoelectric focusing
-
8.1
Taenia solium sequence calculation
-
8.7

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
474
-
(S)-malate pH 8.0, temperature not specified in the publication Taenia solium
474
-
(S)-malate pH 9.6, 22°C, recombinant enzyme Taenia solium
1510
-
oxaloacetate pH 8.0, temperature not specified in the publication Taenia solium
1880
-
NAD+ pH 8.0, temperature not specified in the publication Taenia solium
1880
-
NAD+ pH 9.6, 22°C, recombinant enzyme Taenia solium
5760
-
NADH pH 8.0, temperature not specified in the publication Taenia solium
5760
-
NADH pH 8.0, 22°C, recombinant enzyme Taenia solium
15100
-
oxaloacetate pH 8.0, 22°C, recombinant enzyme Taenia solium