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Literature summary for 1.1.1.37 extracted from
- Enzyme:nanoparticle bioconjugates with two sequential enzymes: stoichiometry and activity of malate dehydrogenase and citrate synthase on Au nanoparticles (2010), Langmuir, 26, 18992-19000.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | adsorption of the enzyme in 30 nm diameter Au nanoparticles for synthesis of bioconjugates, MDH:Au stoichiometry and kinetic parameters determined for MDH:Au, citrate synthase CS:Au, and three types of dual-activity MDH/CS:Au bioconjugates, method development, overview. The number of enzyme molecules adsorbed per particle is dependent upon the enzyme concentration in solution, with multilayers forming at high enzyme:Au solution ratios. The specific activity of adsorbed enzyme increases with increasing number adsorbed per particle for CS:Au, but is less sensitive to stoichiometry for MDH:Au | Sus scrofa |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
oxaloacetate | pH 8.1, 22°C | Sus scrofa |  |
| 0.3 | - |
(S)-malate | pH 8.1, 22°C | Sus scrofa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Sus scrofa | - |
| heart | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 182 | - |
pH 8.1, 22°C, specific activity of free and conjugated enzymes prepared at 300:1 solution enzyme:Au ratio | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malate dehydrogenase | - |
Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature, oxidation and reduction | Sus scrofa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18 | - |
(S)-malate | pH 8.1, 22°C | Sus scrofa | |
| 377 | - |
oxaloacetate | pH 8.1, 22°C | Sus scrofa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.1 | - |
assay at, oxidation and reduction | Sus scrofa |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Sus scrofa | |
| NADH | - |
Sus scrofa | |
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Release 2023.1 (January 2023)
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