KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0076 | - |
oxaloacetate | chloroplast envelope enzyme fraction | Spinacia oleracea | |
0.0081 | - |
oxaloacetate | plasma membrane-bound enzyme fraction | Spinacia oleracea | |
0.0121 | - |
oxaloacetate | chloroplast stroma enzyme fraction | Spinacia oleracea | |
0.0133 | - |
oxaloacetate | intact chloroplast enzyme fraction | Spinacia oleracea | |
0.0381 | - |
NADH | intact chloroplast enzyme fraction | Spinacia oleracea | |
0.0434 | - |
NADH | chloroplast envelope enzyme fraction | Spinacia oleracea | |
0.0459 | - |
oxaloacetate | broken chloroplast enzyme fraction | Spinacia oleracea | |
0.0502 | - |
oxaloacetate | soluble enzyme fraction | Spinacia oleracea | |
0.0729 | - |
NADH | broken chloroplast enzyme fraction | Spinacia oleracea | |
0.0739 | - |
NADH | plasma membrane-bound enzyme fraction | Spinacia oleracea | |
0.078 | - |
NADH | soluble enzyme fraction | Spinacia oleracea | |
0.1172 | - |
NADH | chloroplast stroma enzyme fraction | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Spinacia oleracea | 9507 | - |
chloroplast stroma | - |
Spinacia oleracea | 9570 | - |
chloroplast thylakoid | - |
Spinacia oleracea | 9534 | - |
microsome | - |
Spinacia oleracea | - |
- |
plasma membrane | - |
Spinacia oleracea | 5886 | - |
soluble | - |
Spinacia oleracea | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | the envelope- and plasma membrane-bound NAD-MDH exhibit the highest affinities for OAA, leaf plasma membrane-bound MDH exhibits a high capacity for both reaction directions (malate oxidation and oxaloacetate reduction), while the two chloroplast isoforms (stromal and envelope-bound) preferentially reduce oxaloacetate | Spinacia oleracea | oxaloacetate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD-MDH | - |
Spinacia oleracea |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
soluble and microsomal MDH enzyme activities exhibit closely related properties, with maximal activity at pH 8, chloroplast envelope-bound MDH has maximal activity at pH 8 | Spinacia oleracea |
8.5 | - |
MDH activity in isolated stroma shows an optimum at pH 8.5, leaf plasma membrane-bound activity shows an optimum at pH 8.5 | Spinacia oleracea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Spinacia oleracea |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Spinacia oleracea | the isoform with a pI of 3.5 appears exclusively in the purified plasma membrane isolate, isoelectric focusing | - |
3.5 |
Spinacia oleracea | the remaining soluble isoforms being mainly acidic show a pI between 4 and 5, amongst the acidic isoforms, the most prominent has a pI of around 4.6, isoelectric focusing | - |
4.6 |
Spinacia oleracea | the stromal fraction of the chloroplasts contain one isoform with a pI value around 5.3, isoelectric focusing | - |
5.3 |
Spinacia oleracea | most abundant soluble isoform, isoelectric focusing | - |
9 |