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Literature summary for 1.1.1.37 extracted from

  • Cvetic, T.; Veljovic-Jovanovic, S.; Vucinic, Z.
    Characterization of NAD-dependent malate dehydrogenases from spinach leaves (2008), Protoplasma, 232, 247-253.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
oxaloacetate chloroplast envelope enzyme fraction Spinacia oleracea
0.0081
-
oxaloacetate plasma membrane-bound enzyme fraction Spinacia oleracea
0.0121
-
oxaloacetate chloroplast stroma enzyme fraction Spinacia oleracea
0.0133
-
oxaloacetate intact chloroplast enzyme fraction Spinacia oleracea
0.0381
-
NADH intact chloroplast enzyme fraction Spinacia oleracea
0.0434
-
NADH chloroplast envelope enzyme fraction Spinacia oleracea
0.0459
-
oxaloacetate broken chloroplast enzyme fraction Spinacia oleracea
0.0502
-
oxaloacetate soluble enzyme fraction Spinacia oleracea
0.0729
-
NADH broken chloroplast enzyme fraction Spinacia oleracea
0.0739
-
NADH plasma membrane-bound enzyme fraction Spinacia oleracea
0.078
-
NADH soluble enzyme fraction Spinacia oleracea
0.1172
-
NADH chloroplast stroma enzyme fraction Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
Spinacia oleracea 9507
-
chloroplast stroma
-
Spinacia oleracea 9570
-
chloroplast thylakoid
-
Spinacia oleracea 9534
-
microsome
-
Spinacia oleracea
-
-
plasma membrane
-
Spinacia oleracea 5886
-
soluble
-
Spinacia oleracea
-
-

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ the envelope- and plasma membrane-bound NAD-MDH exhibit the highest affinities for OAA, leaf plasma membrane-bound MDH exhibits a high capacity for both reaction directions (malate oxidation and oxaloacetate reduction), while the two chloroplast isoforms (stromal and envelope-bound) preferentially reduce oxaloacetate Spinacia oleracea oxaloacetate + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
NAD-MDH
-
Spinacia oleracea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
soluble and microsomal MDH enzyme activities exhibit closely related properties, with maximal activity at pH 8, chloroplast envelope-bound MDH has maximal activity at pH 8 Spinacia oleracea
8.5
-
MDH activity in isolated stroma shows an optimum at pH 8.5, leaf plasma membrane-bound activity shows an optimum at pH 8.5 Spinacia oleracea

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Spinacia oleracea

pI Value

Organism Comment pI Value Maximum pI Value
Spinacia oleracea the isoform with a pI of 3.5 appears exclusively in the purified plasma membrane isolate, isoelectric focusing
-
3.5
Spinacia oleracea the remaining soluble isoforms being mainly acidic show a pI between 4 and 5, amongst the acidic isoforms, the most prominent has a pI of around 4.6, isoelectric focusing
-
4.6
Spinacia oleracea the stromal fraction of the chloroplasts contain one isoform with a pI value around 5.3, isoelectric focusing
-
5.3
Spinacia oleracea most abundant soluble isoform, isoelectric focusing
-
9