Crystallization (Comment) | Organism |
---|---|
tetrameric enzyme, hanging drop vapour diffusion method, 15°C, 17 mg/ml protein in 10 mM potassium phosphate buffer, pH 7.0, equilibration of the 0.002 ml protein drop against 500 ml reservoir solution consisting of 1.4 M ammonium sulfate, 5% v/v MPD, 2 mM NAD+, 50 mM sodium citrate buffer, pH 5.5, 2-3 days, X-ray diffraction structure determination and analysis at 1.8 A resolution | Flavobacterium frigidimaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Flavobacterium frigidimaris | malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Flavobacterium frigidimaris KUC-1 | malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle | oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium frigidimaris | Q25QU7 | - |
- |
Flavobacterium frigidimaris KUC-1 | Q25QU7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Flavobacterium frigidimaris | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle | Flavobacterium frigidimaris | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Flavobacterium frigidimaris KUC-1 | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | malate dehydrogenase is an oligomeric enzyme that catalyzes the reversible oxidation of malate to oxaloacetate in the presence of NAD+, MDH is an essential metabolic enzyme in the citric acid cycle | Flavobacterium frigidimaris KUC-1 | oxaloacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Flavobacterium frigidimaris |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Flavobacterium frigidimaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent on | Flavobacterium frigidimaris | |
NADH | dependent on | Flavobacterium frigidimaris |