Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Flavobacterium frigidimaris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
BaCl2 | 1 mM, 24.9% inhibition | Flavobacterium frigidimaris | |
CaCl2 | 1 mM, 29.1% inhibition | Flavobacterium frigidimaris | |
CoCl2 | 1 mM, 39% inhibition | Flavobacterium frigidimaris | |
CuCl2 | 1 mM, 89.8% inhibition | Flavobacterium frigidimaris | |
EDTA | 1 mM, 52.2% inhibition | Flavobacterium frigidimaris | |
HgCl2 | 1 mM, complete inhibition | Flavobacterium frigidimaris | |
hydroxylamine | 1 mM, 57.1% inhibition | Flavobacterium frigidimaris | |
iodoacetate | 1 mM, 93.6% inhibition | Flavobacterium frigidimaris | |
MgCl2 | 1 mM,34.9% inhibition | Flavobacterium frigidimaris | |
MnCl2 | 1 mM, 28.6% inhibition | Flavobacterium frigidimaris | |
NEM | 1 mM, complete inhibition | Flavobacterium frigidimaris | |
NiCl2 | 1 mM, 36.8% inhibition | Flavobacterium frigidimaris | |
Semicarbazide | 1 mM, 61.3% inhibition | Flavobacterium frigidimaris | |
ZnCl2 | 1 mM, 72.6% inhibition | Flavobacterium frigidimaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0279 | - |
NAD+ | 10°C, pH 10.0 | Flavobacterium frigidimaris | |
0.0286 | - |
NAD+ | 20°C, pH 10.0 | Flavobacterium frigidimaris | |
0.0299 | - |
NAD+ | 30°C, pH 10.0 | Flavobacterium frigidimaris | |
0.0382 | - |
NAD+ | 40°C, pH 10.0 | Flavobacterium frigidimaris | |
0.269 | - |
(S)-malate | 20°C, pH 10.0 | Flavobacterium frigidimaris | |
0.288 | - |
(S)-malate | 30°C, pH 10.0 | Flavobacterium frigidimaris | |
0.543 | - |
(S)-malate | 10°C, pH 10.0 | Flavobacterium frigidimaris | |
0.74 | - |
(S)-malate | 40°C, pH 10.0 | Flavobacterium frigidimaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Flavobacterium frigidimaris |
123000 | - |
gel filtration | Flavobacterium frigidimaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium frigidimaris | - |
- |
- |
Flavobacterium frigidimaris KUC-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium frigidimaris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
176 | - |
- |
Flavobacterium frigidimaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | NAD+ binds first to the enzyme and then oxaloacetate is consequently released randomly via an ordered via an ordered bi-bi mechanism | Flavobacterium frigidimaris | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | NAD+ binds first to the enzyme and then oxaloacetate is consequently released randomly via an ordered via an ordered bi-bi mechanism | Flavobacterium frigidimaris KUC-1 | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Flavobacterium frigidimaris | oxaloacetate + NADPH + H+ | - |
ir | |
(S)-malate + NADP+ | - |
Flavobacterium frigidimaris KUC-1 | oxaloacetate + NADPH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Flavobacterium frigidimaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Flavobacterium frigidimaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 55% of maximal activity, 50°C: about 50% of maximal activity | Flavobacterium frigidimaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
half-life: 26 min | Flavobacterium frigidimaris |
40 | - |
half-life: 3 min | Flavobacterium frigidimaris |
45 | - |
half-life: 0.6 min | Flavobacterium frigidimaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.73 | - |
NAD+ | 30°C, pH 10.0 | Flavobacterium frigidimaris | |
41 | - |
NAD+ | 10°C, pH 10.0 | Flavobacterium frigidimaris | |
41.3 | - |
(S)-malate | 10°C, pH 10.0 | Flavobacterium frigidimaris | |
48.4 | - |
(S)-malate | 20°C, pH 10.0 | Flavobacterium frigidimaris | |
48.6 | - |
NAD+ | 20°C, pH 10.0 | Flavobacterium frigidimaris | |
83.2 | - |
(S)-malate | 30°C, pH 10.0 | Flavobacterium frigidimaris | |
83.4 | - |
NAD+ | 30°C, pH 10.0 | Flavobacterium frigidimaris | |
138 | - |
(S)-malate | 40°C, pH 10.0 | Flavobacterium frigidimaris | |
139 | - |
NAD+ | 40°C, pH 10.0 | Flavobacterium frigidimaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reduction of oxaloacetate | Flavobacterium frigidimaris |
10.5 | - |
oxidation of malate | Flavobacterium frigidimaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10.5 | stable | Flavobacterium frigidimaris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the relative activity for NAD+ to that of NADP+ is about 43.9%. pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme | Flavobacterium frigidimaris | |
NADH | pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme | Flavobacterium frigidimaris | |
NADP+ | the relative activity for NAD+ to that of NADP+ is about 43.9%. No activity with NADPH | Flavobacterium frigidimaris |