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Literature summary for 1.1.1.37 extracted from

  • Oikawa, T.; Yamamoto, N.; Shimoke, K.; Uesato, S.; Ikeuchi, T.; Fujioka, T.
    Purification, characterization, and overexpression of psychrophilic and thermolabile malate dehydrogenase of a novel antarctic psychrotolerant, Flavobacterium frigidimaris KUC-1 (2005), Biosci. Biotechnol. Biochem., 69, 2146-2154.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Flavobacterium frigidimaris

Inhibitors

Inhibitors Comment Organism Structure
BaCl2 1 mM, 24.9% inhibition Flavobacterium frigidimaris
CaCl2 1 mM, 29.1% inhibition Flavobacterium frigidimaris
CoCl2 1 mM, 39% inhibition Flavobacterium frigidimaris
CuCl2 1 mM, 89.8% inhibition Flavobacterium frigidimaris
EDTA 1 mM, 52.2% inhibition Flavobacterium frigidimaris
HgCl2 1 mM, complete inhibition Flavobacterium frigidimaris
hydroxylamine 1 mM, 57.1% inhibition Flavobacterium frigidimaris
iodoacetate 1 mM, 93.6% inhibition Flavobacterium frigidimaris
MgCl2 1 mM,34.9% inhibition Flavobacterium frigidimaris
MnCl2 1 mM, 28.6% inhibition Flavobacterium frigidimaris
NEM 1 mM, complete inhibition Flavobacterium frigidimaris
NiCl2 1 mM, 36.8% inhibition Flavobacterium frigidimaris
Semicarbazide 1 mM, 61.3% inhibition Flavobacterium frigidimaris
ZnCl2 1 mM, 72.6% inhibition Flavobacterium frigidimaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0279
-
NAD+ 10°C, pH 10.0 Flavobacterium frigidimaris
0.0286
-
NAD+ 20°C, pH 10.0 Flavobacterium frigidimaris
0.0299
-
NAD+ 30°C, pH 10.0 Flavobacterium frigidimaris
0.0382
-
NAD+ 40°C, pH 10.0 Flavobacterium frigidimaris
0.269
-
(S)-malate 20°C, pH 10.0 Flavobacterium frigidimaris
0.288
-
(S)-malate 30°C, pH 10.0 Flavobacterium frigidimaris
0.543
-
(S)-malate 10°C, pH 10.0 Flavobacterium frigidimaris
0.74
-
(S)-malate 40°C, pH 10.0 Flavobacterium frigidimaris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
4 * 32000, SDS-PAGE Flavobacterium frigidimaris
123000
-
gel filtration Flavobacterium frigidimaris

Organism

Organism UniProt Comment Textmining
Flavobacterium frigidimaris
-
-
-
Flavobacterium frigidimaris KUC-1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Flavobacterium frigidimaris

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
176
-
-
Flavobacterium frigidimaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+ NAD+ binds first to the enzyme and then oxaloacetate is consequently released randomly via an ordered via an ordered bi-bi mechanism Flavobacterium frigidimaris oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+ NAD+ binds first to the enzyme and then oxaloacetate is consequently released randomly via an ordered via an ordered bi-bi mechanism Flavobacterium frigidimaris KUC-1 oxaloacetate + NADH + H+
-
r
(S)-malate + NADP+
-
Flavobacterium frigidimaris oxaloacetate + NADPH + H+
-
ir
(S)-malate + NADP+
-
Flavobacterium frigidimaris KUC-1 oxaloacetate + NADPH + H+
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Flavobacterium frigidimaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Flavobacterium frigidimaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 20°C: about 55% of maximal activity, 50°C: about 50% of maximal activity Flavobacterium frigidimaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
half-life: 26 min Flavobacterium frigidimaris
40
-
half-life: 3 min Flavobacterium frigidimaris
45
-
half-life: 0.6 min Flavobacterium frigidimaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.73
-
NAD+ 30°C, pH 10.0 Flavobacterium frigidimaris
41
-
NAD+ 10°C, pH 10.0 Flavobacterium frigidimaris
41.3
-
(S)-malate 10°C, pH 10.0 Flavobacterium frigidimaris
48.4
-
(S)-malate 20°C, pH 10.0 Flavobacterium frigidimaris
48.6
-
NAD+ 20°C, pH 10.0 Flavobacterium frigidimaris
83.2
-
(S)-malate 30°C, pH 10.0 Flavobacterium frigidimaris
83.4
-
NAD+ 30°C, pH 10.0 Flavobacterium frigidimaris
138
-
(S)-malate 40°C, pH 10.0 Flavobacterium frigidimaris
139
-
NAD+ 40°C, pH 10.0 Flavobacterium frigidimaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
reduction of oxaloacetate Flavobacterium frigidimaris
10.5
-
oxidation of malate Flavobacterium frigidimaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10.5 stable Flavobacterium frigidimaris

Cofactor

Cofactor Comment Organism Structure
NAD+ the relative activity for NAD+ to that of NADP+ is about 43.9%. pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme Flavobacterium frigidimaris
NADH pro-R stereospecificity for hydrogen transfer at the C4 position of the nicotinamide moiety of the coenzyme Flavobacterium frigidimaris
NADP+ the relative activity for NAD+ to that of NADP+ is about 43.9%. No activity with NADPH Flavobacterium frigidimaris