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Literature summary for 1.1.1.37 extracted from

  • Eprintsev, A.T.; Falaleeva, M.I.; Klimova, M.A.; Kompantseva, E.I.
    Physicochemical properties of malate dehydrogenase from the bacterium Rhodopseudomonas palustris strain f8pt (2006), Biochemistry, 71, 692-695.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
NADH pH 8.0, 25°C, tetrameric enzyme form Rhodopseudomonas palustris
0.022
-
oxaloacetate pH 8.0, 25°C, tetrameric enzyme form Rhodopseudomonas palustris
0.023
-
NADH pH 8.0, 25°C, dimeric enzyme form Rhodopseudomonas palustris
0.03
-
oxaloacetate pH 8.0, 25°C, dimeric enzyme form Rhodopseudomonas palustris
0.085
-
NAD+ pH 9.0, 25°C, dimeric enzyme form Rhodopseudomonas palustris
0.27
-
NAD+ pH 9.0, 25°C, tetrameric enzyme form Rhodopseudomonas palustris
0.53
-
(S)-malate pH 9.0, 25°C, dimeric enzyme form Rhodopseudomonas palustris
0.555
-
(S)-malate pH 9.0, 25°C, tetrameric enzyme form Rhodopseudomonas palustris

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, or tetramer, SDS-PAGE Rhodopseudomonas palustris
47000
-
or dimer, 4 * 47000, SDS-PAGE Rhodopseudomonas palustris
90000
-
dimeric enzyme form, gel filtration Rhodopseudomonas palustris
180000
-
tetrameric enzyme form, gel filtration Rhodopseudomonas palustris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodopseudomonas palustris the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle ?
-
?
additional information Rhodopseudomonas palustris f8pt the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle ?
-
?

Organism

Organism UniProt Comment Textmining
Rhodopseudomonas palustris
-
-
-
Rhodopseudomonas palustris f8pt
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.55
-
tetrameric enzyme form Rhodopseudomonas palustris
1.61
-
dimeric enzyme form Rhodopseudomonas palustris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle Rhodopseudomonas palustris ?
-
?
additional information the dimeric isoform of the enzyme is responsible for Krebs cycle function and the tetrameric isoform is involved in functioning of the glyoxylate cycle Rhodopseudomonas palustris f8pt ?
-
?
oxaloacetate + NADH + H+
-
Rhodopseudomonas palustris (S)-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Rhodopseudomonas palustris f8pt (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 47000, or tetramer, SDS-PAGE Rhodopseudomonas palustris
tetramer or dimer, 4 * 47000, SDS-PAGE Rhodopseudomonas palustris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.9
-
reduction of oxaloacetate, dimeric enzyme form Rhodopseudomonas palustris
9
-
reduction of oxaloacetate, tetrameric enzyme form Rhodopseudomonas palustris
9.7
-
oxidation of malate, tetrameric enzyme form Rhodopseudomonas palustris
10.2
-
oxidation of malate, dimeric enzyme form Rhodopseudomonas palustris