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Literature summary for 1.1.1.37 extracted from

  • Eprintsev, A.T.; Falaleeva, M.I.; Klimova, M.A.; Parfenova, N.V.
    Isolation and properties of malate dehydrogenase from meso- and thermophilic bacteria (2006), Appl. Biochem. Microbiol., 42, 241-245.
No PubMed abstract available

Application

Application Comment Organism
analysis use of the thermostable enzyme from Vulcanithermus medioatlanticu DSM 14978 opens broad possibilities for the application of malate dehydrogenase as an analytical reagent and creation on biosensors for biochemical and cliniucal tests Vulcanithermus mediatlanticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0266
-
NADH pH 7.8, 25°C Macromonas bipunctata
0.042
-
NADH pH 7.8, 25°C Vulcanithermus mediatlanticus
0.0476
-
oxaloacetate pH 7.8, 25°C Vulcanithermus mediatlanticus
0.053
-
oxaloacetate pH 7.8, 25°C Macromonas bipunctata
0.263
-
NAD+ pH 9.0, 25°C Macromonas bipunctata
0.4
-
NAD+ pH 9.0, 25°C Vulcanithermus mediatlanticus
0.645
-
(S)-malate pH 9.0, 25°C Macromonas bipunctata
1.67
-
(S)-malate pH 9.0, 25°C Vulcanithermus mediatlanticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
2 * 45000, SDS-PAGE Macromonas bipunctata
58000
-
2 * 58000, SDS-PAGE Vulcanithermus mediatlanticus
92000
-
gel filtration Macromonas bipunctata
128000
-
gel filtration Vulcanithermus mediatlanticus

Organism

Organism UniProt Comment Textmining
Macromonas bipunctata
-
-
-
Macromonas bipunctata D-405
-
-
-
Vulcanithermus mediatlanticus
-
DSM 14978
-

Purification (Commentary)

Purification (Comment) Organism
-
Macromonas bipunctata
-
Vulcanithermus mediatlanticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5
-
-
Vulcanithermus mediatlanticus
26.9
-
-
Macromonas bipunctata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Macromonas bipunctata (S)-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Vulcanithermus mediatlanticus (S)-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Macromonas bipunctata D-405 (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 45000, SDS-PAGE Macromonas bipunctata
dimer 2 * 58000, SDS-PAGE Vulcanithermus mediatlanticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
53
-
-
Macromonas bipunctata
82
-
-
Vulcanithermus mediatlanticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
47
-
complete inactivation Macromonas bipunctata
50
-
15 min, stable Vulcanithermus mediatlanticus
60
-
15 min, about 25% loss of activity Vulcanithermus mediatlanticus
70
-
15 min, about 50% loss of activity Vulcanithermus mediatlanticus
80
-
15 min, about 70% loss of activity Vulcanithermus mediatlanticus
85
-
1 min, complete loss of activity Vulcanithermus mediatlanticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
reduction of oxaloacetate Vulcanithermus mediatlanticus
7.7
-
reduction of oxaloacetate Macromonas bipunctata
8.5
-
oxidation of malate Vulcanithermus mediatlanticus
10.2
-
oxidation of malate Macromonas bipunctata

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Macromonas bipunctata
NAD+
-
Vulcanithermus mediatlanticus
NADH
-
Macromonas bipunctata
NADH
-
Vulcanithermus mediatlanticus