General Stability | Organism |
---|---|
the enzyme is sensitive to proteolytic attack | Mesembryanthemum cordifolium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
oxaloacetate | substrate inhibition, pH-dependent | Mesembryanthemum cordifolium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0104 | - |
NADH | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
0.017 | - |
NADH | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
0.0391 | - |
oxaloacetate | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
0.111 | - |
oxaloacetate | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
0.1338 | - |
NAD+ | pH 7.5, 30°C | Mesembryanthemum cordifolium | |
0.1397 | - |
NAD+ | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
2.068 | - |
L-malate | pH 6.9, 30°C | Mesembryanthemum cordifolium | |
2.28 | - |
L-malate | pH 7.5, 30°C | Mesembryanthemum cordifolium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39400 | - |
2 * 39400, SDS-PAGE | Mesembryanthemum cordifolium |
83000 | - |
gel filtration | Mesembryanthemum cordifolium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mesembryanthemum cordifolium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mesembryanthemum cordifolium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Mesembryanthemum cordifolium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1740 | - |
- |
Mesembryanthemum cordifolium |
Storage Stability | Organism |
---|---|
-80°C, stable for 4 months | Mesembryanthemum cordifolium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH | - |
Mesembryanthemum cordifolium | L-malate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39400, SDS-PAGE | Mesembryanthemum cordifolium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
reduction of oxaloacetate | Mesembryanthemum cordifolium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 75 | 35°C: about 40% of maximal activity, 75°C: about 90% of maximal activity, reduction of oxaloacetate | Mesembryanthemum cordifolium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Mesembryanthemum cordifolium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reduction of oxaloacetate | Mesembryanthemum cordifolium |
7.2 | 8.4 | oxidation of malate | Mesembryanthemum cordifolium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | pH 6.0: about 45% of maximal activity, pH 8.0: about 50% of maximal activity, reduction of oxaloacetate | Mesembryanthemum cordifolium |
6.8 | 9 | pH 6.8: about 40% of maximal activity, pH 9.0: about 65% of maximal activity, oxidation of malate | Mesembryanthemum cordifolium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Mesembryanthemum cordifolium | |
NADH | - |
Mesembryanthemum cordifolium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.23 | - |
oxaloacetate | pH 6.9 | Mesembryanthemum cordifolium | |
0.6 | - |
oxaloacetate | pH 7.5 | Mesembryanthemum cordifolium |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mesembryanthemum cordifolium | denaturing isoelectric focusing | - |
6 |