Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.37 extracted from

  • Maloney, A.P.; Callan, S.M.; Murray, P.G.; Tuohy, M.G.
    Mitochondrial malate dehydrogenase from the thermophilic, filamentous fungus Talaromyces emersonii (2004), Eur. J. Biochem., 271, 3115-3126.
    View publication on PubMed
Search for more literature for 1.1.1.37

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as a His-tagged protein Rasamsonia emersonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 oxaloacetate pH 7.4, 30°C, native enzyme Rasamsonia emersonii
0.02
-
 oxaloacetate native enzyme, at pH 7.4 and 30°C Rasamsonia emersonii
0.022
-
 oxaloacetate pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
0.022
-
 oxaloacetate recombinant enzyme, at pH 7.4 and 30°C Rasamsonia emersonii
0.025
-
 NADH pH 7.4, 30°C, native enzyme Rasamsonia emersonii
0.025
-
 NADH native enzyme, at pH 7.4 and 30°C Rasamsonia emersonii
0.029
-
 NADH pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
0.029
-
 NADH recombinant enzyme, at pH 7.4 and 30°C Rasamsonia emersonii
0.12
-
 NAD+ pH 7.4, 30°C, native enzyme Rasamsonia emersonii
0.12
-
 NAD+ native enzyme, at pH 7.4 and 30°C Rasamsonia emersonii
0.13
-
 NAD+ pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
0.13
-
 NAD+ recombinant enzyme, at pH 7.4 and 30°C Rasamsonia emersonii
1
-
 L-malate pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
1.25
-
 L-malate pH 7.4, 30°C, native enzyme Rasamsonia emersonii

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rasamsonia emersonii 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Rasamsonia emersonii
70000
-
 gel filtration Rasamsonia emersonii

Organism

Organism UniProt Comment Textmining
Rasamsonia emersonii Q8TG27 mitochondrial malate dehydrogenase cDNA sequence
-
Rasamsonia emersonii Q8X1C8 m-MDH chromosomal DNA sequence
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rasamsonia emersonii

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1610
-
-
 Rasamsonia emersonii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
 Rasamsonia emersonii oxaloacetate + NADH + H+
-
 r
oxaloacetate + NADH
-
 Rasamsonia emersonii L-malate + NAD+
-
 ?
oxaloacetate + NADH + H+
-
 Rasamsonia emersonii (S)-malate + NAD+
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Rasamsonia emersonii

Synonyms

Synonyms Comment Organism
m-MDH
-
 Rasamsonia emersonii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 56 reduction of oxaloacetate, recombinant enzyme Rasamsonia emersonii
52
-
 reduction of oxaloacetate, native enzyme Rasamsonia emersonii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 complete inactivation of native enzyme after 1 h, complete inactivation of recombinant enzyme after 15 min, half-life is 30 min in absence of bovine serum albumin, less than 10% loss of activity after 30 min in presence of 0.2 mg/ml bovine serum albumin Rasamsonia emersonii
60
-
 10 min, complete inactivation Rasamsonia emersonii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2770
-
 NAD+ pH 7.4, 30°C, native enzyme Rasamsonia emersonii
2900
-
 L-malate pH 7.4, 30°C, native enzyme Rasamsonia emersonii
3100
-
 NAD+ pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
3500
-
 L-malate pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
81000
-
 oxaloacetate pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
90000
-
 NADH pH 7.4, 30°C, recombinant enzyme Rasamsonia emersonii
91000
-
 oxaloacetate pH 7.4, 30°C, native enzyme Rasamsonia emersonii
110000
-
 NADH pH 7.4, 30°C, native enzyme Rasamsonia emersonii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 reduction of oxaloacetate, native enzyme and recombinant enzyme Rasamsonia emersonii
10
-
 oxidation of malate, native enzyme and recombinant enzyme Rasamsonia emersonii

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Rasamsonia emersonii