Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-malate | inhibition constant: 3.65 mM | Beggiatoa leptomitoformis | |
Zn2+ | inhibition constant 1.7 mM | Beggiatoa leptomitoformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADH | 25°C | Beggiatoa leptomitoformis | |
0.02 | - |
oxaloacetate | 25°C | Beggiatoa leptomitoformis | |
0.53 | - |
NAD+ | 25°C | Beggiatoa leptomitoformis | |
0.67 | - |
L-malate | 25°C | Beggiatoa leptomitoformis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | activation constant: 4.1 mM | Beggiatoa leptomitoformis | |
Ca2+ | activation constant: 3.3 mM | Beggiatoa leptomitoformis | |
Mg2+ | activation constant: 2.5 mM | Beggiatoa leptomitoformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Beggiatoa leptomitoformis |
172000 | - |
gel filtration | Beggiatoa leptomitoformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Beggiatoa leptomitoformis | the enzyme is involved in the tricarboxylic acid cycle supplying organic acids as the main source of carbon but not energy | ? | - |
? | |
additional information | Beggiatoa leptomitoformis D-402 | the enzyme is involved in the tricarboxylic acid cycle supplying organic acids as the main source of carbon but not energy | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beggiatoa leptomitoformis | - |
- |
- |
Beggiatoa leptomitoformis D-402 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Beggiatoa leptomitoformis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.43 | - |
- |
Beggiatoa leptomitoformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Beggiatoa leptomitoformis | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Beggiatoa leptomitoformis D-402 | oxaloacetate + NADH + H+ | - |
r | |
additional information | the enzyme is involved in the tricarboxylic acid cycle supplying organic acids as the main source of carbon but not energy | Beggiatoa leptomitoformis | ? | - |
? | |
additional information | the enzyme is involved in the tricarboxylic acid cycle supplying organic acids as the main source of carbon but not energy | Beggiatoa leptomitoformis D-402 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Beggiatoa leptomitoformis |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Beggiatoa leptomitoformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
oxidation of L-malate | Beggiatoa leptomitoformis |
55 | - |
reduction of oxaloacetate | Beggiatoa leptomitoformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
reduction of oxaloacetate | Beggiatoa leptomitoformis |
10.2 | - |
oxidation of L-malate | Beggiatoa leptomitoformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Beggiatoa leptomitoformis | |
NADH | - |
Beggiatoa leptomitoformis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
Zn2+ | - |
Beggiatoa leptomitoformis | |
3.65 | - |
L-malate | - |
Beggiatoa leptomitoformis |