Crystallization (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
in presence of ethylene glycol 4000 | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-malate | product inhibition, 66% inhibition at 5 mM | Sulfolobus acidocaldarius | |
2-oxoglutarate | 44% inhibition at 5 mM | Sulfolobus acidocaldarius | |
alpha-ketoglutarate | - |
Methanospirillum hungatei | |
alpha-ketoglutarate | - |
Sulfolobus acidocaldarius | |
L-malate | - |
Methanospirillum hungatei | |
L-malate | product inhibition | Sulfolobus acidocaldarius | |
L-malate | - |
Sus scrofa | |
additional information | not inhibitory: AMP, ADP, ATP | Sulfolobus acidocaldarius | |
NAD+ | product inhibition; product inhibition, 29% inhibition at 5 mM, 42% inhibition at 10 mM | Sulfolobus acidocaldarius | |
NADP+ | product inhibition; product inhibition, 26% inhibition at 10 mM | Sulfolobus acidocaldarius | |
oxaloacetate | substrate inhibition; substrate inhibition, 29% inhibition at 5 mM | Sulfolobus acidocaldarius | |
oxaloacetate | - |
Sus scrofa | |
oxaloacetate | substrate inhibition | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
NADH | - |
Sulfolobus acidocaldarius | |
0.0041 | - |
NADH | 40°C, pH 5.8 | Sulfolobus acidocaldarius | |
0.052 | - |
oxaloacetate | - |
Sulfolobus acidocaldarius | |
0.052 | - |
oxaloacetate | 40°C, pH 5.8 | Sulfolobus acidocaldarius | |
0.2 | - |
NADPH | - |
Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
4 * 34000, SDS-PAGE | Sulfolobus acidocaldarius |
97000 | - |
gel filtration | Sulfolobus acidocaldarius |
128500 | - |
ultracentrifugation | Sulfolobus acidocaldarius |
128500 | - |
ultracentrifugation, sedimentation equilibrium studies | Sulfolobus acidocaldarius |
134000 | - |
cross-linking data | Sulfolobus acidocaldarius |
134000 | - |
SDS-PAGE of enzyme after cross-linking | Sulfolobus acidocaldarius |
140000 | - |
- |
Laceyella sacchari |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malate + NAD+ | Thermus aquaticus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Thermus thermophilus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Escherichia coli | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Sus scrofa | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Methanothermobacter thermautotrophicus | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Sulfolobus acidocaldarius | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Thermoplasma acidophilum | - |
oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | Laceyella sacchari | - |
oxaloacetate + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Laceyella sacchari | - |
- |
- |
Methanospirillum hungatei | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Sulfolobus acidocaldarius | - |
- |
- |
Sus scrofa | - |
pig | - |
Thermoplasma acidophilum | - |
- |
- |
Thermus aquaticus | - |
- |
- |
Thermus thermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
L-malate oxidation in presence of NAD+ | Sulfolobus acidocaldarius |
277 | - |
- |
Sulfolobus acidocaldarius |
277 | - |
40°C, pH 5.8 | Sulfolobus acidocaldarius |
Storage Stability | Organism |
---|---|
-20°C, can be stored over months | Sulfolobus acidocaldarius |
4°C, pH 7.0-9.0, concentration of 0.0018 mg/ml, quite stable. Below pH 7.0, inactivation occurs | Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Thermus aquaticus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Thermus thermophilus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Methanothermobacter thermautotrophicus | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Sulfolobus acidocaldarius | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Thermoplasma acidophilum | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Laceyella sacchari | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | low activity in this direction | Sulfolobus acidocaldarius | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Thermus aquaticus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Thermus thermophilus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Escherichia coli | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Sus scrofa | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Methanothermobacter thermautotrophicus | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Sulfolobus acidocaldarius | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Thermoplasma acidophilum | oxaloacetate + NADH + H+ | - |
r | |
malate + NAD+ | - |
Laceyella sacchari | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH | - |
Thermus aquaticus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Thermus thermophilus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Escherichia coli | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Sus scrofa | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Methanothermobacter thermautotrophicus | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Sulfolobus acidocaldarius | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Thermoplasma acidophilum | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH | - |
Methanospirillum hungatei | L-malate + NAD+ | - |
? | |
oxaloacetate + NADH | - |
Laceyella sacchari | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Sulfolobus acidocaldarius | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADPH + H+ | - |
Sulfolobus acidocaldarius | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Escherichia coli |
tetramer | - |
Thermoplasma acidophilum |
tetramer | - |
Laceyella sacchari |
tetramer | 4 * 34000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
8 | 80 | - |
Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
activity of purified enzyme decreases continually from 4°C to 80°C | Sulfolobus acidocaldarius |
75 | - |
15 min, 75% residual activity, purified enzyme | Sulfolobus acidocaldarius |
80 | - |
15 min, no loss of activity, enzyme in crude extract | Sulfolobus acidocaldarius |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.62 | - |
oxaloacetate | - |
Sulfolobus acidocaldarius |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | - |
complete inactivation | Sulfolobus acidocaldarius |
5.5 | - |
24 h, 50% loss of activity | Sulfolobus acidocaldarius |
7 | - |
inactivation below | Sulfolobus acidocaldarius |
7 | 9 | quite stable | Sulfolobus acidocaldarius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sulfolobus acidocaldarius | |
NAD+ | - |
Thermoplasma acidophilum | |
NADH | A-side stereospecificity | Sulfolobus acidocaldarius | |
NADP+ | - |
Sulfolobus acidocaldarius | |
NADP+ | - |
Thermoplasma acidophilum | |
NADPH | A-side stereospecificity | Sulfolobus acidocaldarius |