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Literature summary for 1.1.1.37 extracted from

  • Waters, J.K.; Karr, D.B.; Emerich, D.W.
    Malate dehydrogenase from Rhizobium japonicum 3I1b-143 bacteroids and Glycine max root-nodule mitochondria (1985), Biochemistry, 24, 6479-6486.
No PubMed abstract available

General Stability

General Stability Organism
mitochondrial malate dehydrogenase is very unstable Glycine max

Inhibitors

Inhibitors Comment Organism Structure
Dicarbonate
-
Bradyrhizobium japonicum
Dicarbonate
-
Glycine max
iodoacetamide
-
Glycine max
Phenylmethanesulfonylfluoride
-
Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Euglena gracilis 5829
-
cytosol
-
Bradyrhizobium japonicum 5829
-
cytosol
-
Glycine max 5829
-
mitochondrion
-
Euglena gracilis 5739
-
mitochondrion
-
Bradyrhizobium japonicum 5739
-
mitochondrion
-
Glycine max 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
4 * 36000, SDS-PAGE Bradyrhizobium japonicum
37000
-
1 * 39000 + 1 * 37000, SDS-PAGE Glycine max
39000
-
1 * 39000 + 1 * 37000, SDS-PAGE Glycine max
69000
-
-
Euglena gracilis
69000
-
m-MDH Glycine max
69400
-
m-MDH Euglena gracilis
69400
-
m-MDH Glycine max
138600
-
-
Bradyrhizobium japonicum
139000
-
gel filtration Bradyrhizobium japonicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Phaseolus vulgaris
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Bacillus subtilis
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Escherichia coli
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Euglena gracilis
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Geobacillus stearothermophilus
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Bradyrhizobium japonicum
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Pisum sativum
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Zea mays
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Glycine max
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Chromobacterium violaceum
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bradyrhizobium japonicum
-
3I1B-143 bacteroids
-
Chromobacterium violaceum
-
-
-
Escherichia coli
-
-
-
Euglena gracilis
-
-
-
Geobacillus stearothermophilus
-
-
-
Glycine max
-
soybean, Williams 79 cultivar
-
Phaseolus vulgaris
-
-
-
Pisum sativum
-
pea
-
Zea mays
-
maize
-

Purification (Commentary)

Purification (Comment) Organism
-
Bradyrhizobium japonicum
-
Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
bacteroid
-
Bradyrhizobium japonicum
-
leaf
-
Pisum sativum
-
root
-
Pisum sativum
-
root nodule Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1270
-
-
Bradyrhizobium japonicum
1675
-
-
Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Phaseolus vulgaris oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Euglena gracilis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Bradyrhizobium japonicum oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Pisum sativum oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Zea mays oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Glycine max oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Chromobacterium violaceum oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Phaseolus vulgaris oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Euglena gracilis oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Geobacillus stearothermophilus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Bradyrhizobium japonicum oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Pisum sativum oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Zea mays oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Glycine max oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Chromobacterium violaceum oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH
-
Phaseolus vulgaris L-malate + NAD+
-
r
oxaloacetate + NADH
-
Bacillus subtilis L-malate + NAD+
-
r
oxaloacetate + NADH
-
Escherichia coli L-malate + NAD+
-
r
oxaloacetate + NADH
-
Euglena gracilis L-malate + NAD+
-
r
oxaloacetate + NADH
-
Geobacillus stearothermophilus L-malate + NAD+
-
r
oxaloacetate + NADH
-
Bradyrhizobium japonicum L-malate + NAD+
-
r
oxaloacetate + NADH
-
Pisum sativum L-malate + NAD+
-
r
oxaloacetate + NADH
-
Zea mays L-malate + NAD+
-
r
oxaloacetate + NADH
-
Glycine max L-malate + NAD+
-
r
oxaloacetate + NADH
-
Chromobacterium violaceum L-malate + NAD+
-
r

Subunits

Subunits Comment Organism
dimer
-
Euglena gracilis
dimer
-
Bradyrhizobium japonicum
dimer 1 * 39000 + 1 * 37000, SDS-PAGE Glycine max
tetramer
-
Bacillus subtilis
tetramer
-
Escherichia coli
tetramer
-
Geobacillus stearothermophilus
tetramer 4 * 36000, SDS-PAGE Bradyrhizobium japonicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
stable for 20 min, loses more than 95% activity when subjected to 55°C for the same period of time Bacillus subtilis
50
-
stable for 20 min, loses more than 95% activity when subjected to 55°C for the same period of time Escherichia coli
75
-
stable up to, but loses all activity when heated to 80°C for 20 min Geobacillus stearothermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.3 conversion of oxaloacetate to L-malate Glycine max
8 8.5 conversion of oxaloacetate to L-malate Bradyrhizobium japonicum
8.2 8.6 conversion of L-malate to oxaloacetate Glycine max
8.6 9 conversion of L-malate to oxaloacetate Bradyrhizobium japonicum