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Literature summary for 1.1.1.37 extracted from

  • Smith, K.; Sundaram, T.K.; Kernick, M.; Wilkinson, A.E.
    Purification of bacterial malate dehydrogenases by selective elution from a triazinyl dye affinity column (1982), Biochim. Biophys. Acta, 708, 17-25.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malate + NAD+ Thermus aquaticus
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Bacillus subtilis
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Escherichia coli
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Bacillus sp. (in: Bacteria)
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ [Bacillus] caldotenax
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Comamonas testosteroni
-
oxaloacetate + NADH + H+
-
r
malate + NAD+ Thermus aquaticus B
-
oxaloacetate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
moderate thermophile, designated BI
-
Bacillus subtilis
-
-
-
Comamonas testosteroni
-
-
-
Escherichia coli
-
-
-
Thermus aquaticus
-
-
-
Thermus aquaticus B
-
-
-
[Bacillus] caldotenax
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus aquaticus
-
Bacillus subtilis
-
Escherichia coli
-
[Bacillus] caldotenax

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
83
-
-
[Bacillus] caldotenax
100.5
-
-
Thermus aquaticus
187
-
-
Bacillus sp. (in: Bacteria)
322
-
-
Bacillus subtilis
1920
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Thermus aquaticus oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Bacillus sp. (in: Bacteria) oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
[Bacillus] caldotenax oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Comamonas testosteroni oxaloacetate + NADH + H+
-
r
(S)-malate + NAD+
-
Thermus aquaticus B oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Thermus aquaticus oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Bacillus subtilis oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Escherichia coli oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Bacillus sp. (in: Bacteria) oxaloacetate + NADH + H+
-
r
malate + NAD+
-
[Bacillus] caldotenax oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Comamonas testosteroni oxaloacetate + NADH + H+
-
r
malate + NAD+
-
Thermus aquaticus B oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH
-
Thermus aquaticus L-malate + NAD+
-
r
oxaloacetate + NADH
-
Bacillus subtilis L-malate + NAD+
-
r
oxaloacetate + NADH
-
Escherichia coli L-malate + NAD+
-
r
oxaloacetate + NADH
-
Bacillus sp. (in: Bacteria) L-malate + NAD+
-
r
oxaloacetate + NADH
-
[Bacillus] caldotenax L-malate + NAD+
-
r
oxaloacetate + NADH
-
Comamonas testosteroni L-malate + NAD+
-
r
oxaloacetate + NADH
-
Thermus aquaticus B L-malate + NAD+
-
r