Literature summary for 1.1.1.365 extracted from

Amaya, I.; Osorio, S.; Martinez-Ferri, E.; Lima-Silva, V.; Doblas, V.G.; Fernandez-Munoz, R.; Fernie, A.R.; Botella, M.A.; Valpuesta, V.
Increased antioxidant capacity in tomato by ectopic expression of the strawberry D-galacturonate reductase gene (2015), Biotechnol. J., 10, 490-500 .

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Cloned(Commentary)

Cloned (Comment)	Organism
ORF FaGalUR is recombinantly expressed in Solanum lycopersicum from plasmid pPGGUSI, which contains a 4.8 Kb promoter fragment of the polygalacturonase (PG) gene and its terminator, or from a plasmid containing the 35S CaMV promoter, mobilized from Escherichia coli by triparental mating, via Agrobacterium tumefaciens strain LBA4404 in tomato plants, PCR expression analysis. FaGalUR expression driven by the CaMV 35S promoter causes the expression of the gene in most parts of the plants, while in the second, FaGalU, is expressed under the control of the tomato fruit ripening-specific PG promoter. All of the transgenic lines are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. No significant changes in soluble solids of transgenic plants, but a reduction in acidity	Fragaria x ananassa

Cloned (Comment)

Organism

ORF FaGalUR is recombinantly expressed in Solanum lycopersicum from plasmid pPGGUSI, which contains a 4.8 Kb promoter fragment of the polygalacturonase (PG) gene and its terminator, or from a plasmid containing the 35S CaMV promoter, mobilized from Escherichia coli by triparental mating, via Agrobacterium tumefaciens strain LBA4404 in tomato plants, PCR expression analysis. FaGalUR expression driven by the CaMV 35S promoter causes the expression of the gene in most parts of the plants, while in the second, FaGalU, is expressed under the control of the tomato fruit ripening-specific PG promoter. All of the transgenic lines are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. No significant changes in soluble solids of transgenic plants, but a reduction in acidity

Fragaria x ananassa

Organism

Organism	UniProt	Comment	Textmining
Fragaria x ananassa	O49133	Fragaria chiloensis x Fragaria virginiana	-

Synonyms

Synonyms	Comment	Organism
AKR2	-	Fragaria x ananassa
D-galacturonate reductase	-	Fragaria x ananassa
FaGalUR	ORF name	Fragaria x ananassa
GalUR	-	Fragaria x ananassa

General Information

General Information	Comment	Organism
physiological function	all of transgenic Solanum lycopersicum lines overexpressing the Fragaria ananassa D-galacturonic acid reductase gene are morphologically indistinguishable over different generations from control lines both in vegetative traits, such as leaf size or plant height, and fruit traits such as color or size. The majority of transgenic plants display a slight increase in fruit yield, up to 1.4fold, which is a consequence of an increase in the number of fruits rather than an increase in fruit weight. The plants show no significant changes in soluble solids of transgenic plants, but a reduction in acidity. Transgenic lines show a moderate increase on AsA content, and complex changes in metabolites are found in transgenic fruits. Phenotypes, overview	Fragaria x ananassa