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Literature summary for 1.1.1.365 extracted from

  • Zhang, L.; Thiewes, H.; van Kan, J.A.
    The D-galacturonic acid catabolic pathway in Botrytis cinerea (2011), Fungal Genet. Biol., 48, 990-997.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Botrytis cinerea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
D-glucuronate isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea
1
-
D-glucuronate isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea
1.4
-
D-galacturonate isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea
2.5
-
D-galacturonate isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C Botrytis cinerea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36900
-
x * 36900, isoform GAR1, calculated from amino acid sequence Botrytis cinerea
49200
-
x * 49200, isoform GAR2, calculated from amino acid sequence Botrytis cinerea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonate + NADPH + H+ Botrytis cinerea
-
L-galactonate + NADP+
-
r
D-galacturonate + NADPH + H+ Botrytis cinerea B05.10
-
L-galactonate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Botrytis cinerea
-
-
-
Botrytis cinerea B05.10
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NADPH + H+
-
Botrytis cinerea L-galactonate + NADP+
-
r
D-galacturonate + NADPH + H+
-
Botrytis cinerea B05.10 L-galactonate + NADP+
-
r
D-glucuronate + NADPH + H+
-
Botrytis cinerea ?
-
r
D-glucuronate + NADPH + H+
-
Botrytis cinerea B05.10 ?
-
r

Subunits

Subunits Comment Organism
? x * 36900, isoform GAR1, calculated from amino acid sequence Botrytis cinerea
? x * 49200, isoform GAR2, calculated from amino acid sequence Botrytis cinerea

Synonyms

Synonyms Comment Organism
gar1 isoform Botrytis cinerea
GAR2 isoform Botrytis cinerea

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Botrytis cinerea

Expression

Organism Comment Expression
Botrytis cinerea isoform GAR1 is induced about 10fold, while isoform GAR2 is strongly induced in cultures with D-galacturonic acid, pectate, and pectin as carbon source at 3 h after transfer, compared to continuous growth in glucose-containing culture up