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Literature summary for 1.1.1.346 extracted from
- Purification and identification of an Escherichia coli beta-keto ester reductase as 2,5-diketo-D-gluconate reductase YqhE (2002), Biotechnol. Prog., 18, 257-261.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.1 | - |
ethyl 2-methylacetoacetate | at pH 7.0 and 30°C | Escherichia coli |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31800 | - |
x * 31800, estimated from SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli BL21-(DE3) | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Matrex Red-A column chromatography, Sephacryl S-200 gel filtration, and hypatite C cholumn chromatography | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.3 | - |
purified enzyme, at pH 7.0 and 30°C | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, potassium buffer, several weeks, no loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethyl 2-acetylpent-4-enoate + NADPH + H+ | 250% activity compared to ethyl 2-methylacetoacetate | Escherichia coli | ethyl (2R)-2-[(1S)-1-hydroxyethyl]pent-4-enoate + NADP+ | - |
? | |
| ethyl 2-ethyl-3-oxobutanoate + NADPH + H+ | 120% activity compared to ethyl 2-methylacetoacetate | Escherichia coli | ethyl (2R,3S)-2-ethyl-3-hydroxybutanoate + NADP+ | - |
? | |
| ethyl 2-methylacetoacetate + NADH + H+ | 7% activity with NADH compared to NADPH | Escherichia coli | ethyl (2R)-methyl-(3S)-hydroxybutanoate + NAD+ | - |
? | |
| ethyl 2-methylacetoacetate + NADPH + H+ | - |
Escherichia coli | ethyl (2R)-methyl-(3S)-hydroxybutanoate + NADP+ | - |
? | |
| ethyl acetoacetate + NADPH + H+ | 53% activity compared to ethyl 2-methylacetoacetate | Escherichia coli | ethyl (3S)-3-hydroxybutanoate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 31800, estimated from SDS-PAGE | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 2,5-diketo-D-gluconate reductase | - |
Escherichia coli |
| 2,5-DKG reductase | - |
Escherichia coli |
| YqhE | - |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.6 | - |
ethyl 2-methylacetoacetate | at pH 7.0 and 30°C | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | dependent on | Escherichia coli | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Escherichia coli | isoelectric focusing | - |
5.9 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | the enzyme reduces 2,5-didehydro-D-gluconate, a key step in the microbial synthesis of vitamin C | Escherichia coli |
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