Protein Variants | Comment | Organism |
---|---|---|
F22Y/A272G | substrate-binding pocket double mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
F22Y/K232G/R235G/R238H/A272G | mutant with wild type kcat value for NADPH | Corynebacterium sp. |
F22Y/K232G/R235T/R238H/A272G 420 | mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
F22Y/K232G/R238H/A272G | mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
F22Y/K232G/R238H/A272G | the mutant exhibits activity with NADH that is more than 2 orders of magnitude higher than that of the wild type enzyme and retains a high level of activity with NADPH | Corynebacterium sp. |
K232G/R238H | mutant with decreased kcat value for NADPH compared to the wild type enzyme | Corynebacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADPH + H+ | - |
Corynebacterium sp. | 2-dehydro-L-gulonate + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconic acid reductase | - |
Corynebacterium sp. |
2,5-DKG reductase | - |
Corynebacterium sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
NADPH | mutant enzyme F22Y/S233T/R235S/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
3.3 | - |
NADPH | mutant enzyme F22Y/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
7 | - |
NADPH | mutant enzyme F22Y/K232G/R235G/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
7.3 | - |
NADPH | mutant enzyme K232G/R238H, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
18.3 | - |
NADPH | mutant enzyme F22Y/K232G/R238H/A272G, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. | |
18.3 | - |
NADPH | wild type enzyme, in 100 mM Bis-Tris, pH 7.0, at 25°C | Corynebacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | the enzyme has a preference for NADPH over NADH | Corynebacterium sp. |