Cloned (Comment) | Organism |
---|---|
mutant enzymes are expressed in Escherichia coli JM109 cells | Corynebacterium sp. |
Protein Variants | Comment | Organism |
---|---|---|
K233G | the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme | Corynebacterium sp. |
K233H | the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
K233M | the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme | Corynebacterium sp. |
K233Q | the mutant shows wild type NADPH activity and increased NADH activity | Corynebacterium sp. |
K233R | the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
K233S | the mutant shows wild type NADPH activity and increased NADH activity compared to the wild type enzyme | Corynebacterium sp. |
K233T | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235C | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235D | the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
R235E | the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
R235G | the mutant shows decreased NADPH activity and increased NADH activity compared to the wild type enzyme | Corynebacterium sp. |
R235H | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235M | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235N | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235Q | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235S | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R235T | the mutant shows wild type NADPH activity and increased NADH activity | Corynebacterium sp. |
R235Y | the mutant shows reduced NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
R238D | the mutant shows no activity with NADPH and NADH | Corynebacterium sp. |
R238E | the mutant shows no activity with NADPH and increased NADH activity compared to the wild type enzyme | Corynebacterium sp. |
R238F | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
R238G | the mutant shows reduced NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
R238H | the mutant shows wild type NADPH activity and increased NADH activity | Corynebacterium sp. |
R238N | the mutant shows reduced NADPH activity and no NADH activity | Corynebacterium sp. |
R238Q | the mutant shows reduced NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
R238Y | the mutant shows reduced NADPH activity and increased NADH activity ompared to the wild type enzyme | Corynebacterium sp. |
S233E | the mutant shows no activity with NADPH and NADH | Corynebacterium sp. |
S233K | the mutant shows no activity with NADPH and NADH | Corynebacterium sp. |
S233M | the mutant shows no activity with NADPH and NADH | Corynebacterium sp. |
S233N | the mutant shows no activity with NADPH and NADH | Corynebacterium sp. |
S233T | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
S233V | the mutant shows no activity with NADPH and NADH | Corynebacterium sp. |
V234D | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234E | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234I | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234M | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234M/R235C | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234N | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234Q | the mutant shows wild type NADPH activity and no NADH activity | Corynebacterium sp. |
V234S | the mutant shows decreased NADPH activity compared to the wild type enzyme and no NADH activity | Corynebacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
1 * 34000, SDS-PAGE | Corynebacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE cellulose resin column chromatography, gel filtration | Corynebacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-didehydro-D-gluconate + NADPH + H+ | - |
Corynebacterium sp. | 2-dehydro-L-gulonate + NADP+ | - |
? | |
additional information | the wild type enzyme shows no activity with NADH | Corynebacterium sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 34000, SDS-PAGE | Corynebacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
2,5-diketo-D-gluconic acid reductase A | - |
Corynebacterium sp. |
2,5-DKG reductase | - |
Corynebacterium sp. |
AKR5C | - |
Corynebacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | dependent on | Corynebacterium sp. |