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Literature summary for 1.1.1.346 extracted from

  • Yum, D.Y.; Lee, B.Y.; Pan, J.G.
    Identification of the yqhE and yafB genes encoding two 2,5-diketo-D-gluconate reductases in Escherichia coli (1999), Appl. Environ. Microbiol., 65, 3341-3346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.346

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 1 * 29000, SDS-PAGE Escherichia coli
30000
-
 gel filtration Escherichia coli
31003
-
 1 * 31003, calculated from amino acid sequence Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli Q46857
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+ NADPH is the preferred electron donor Escherichia coli 2-dehydro-L-gulonate + NADP+
-
 ?
additional information 2-keto-D-gluconate, 2-keto-L-gulonate, 5-keto-D-gluconate, D-fructose, and L-sorbose are inactive when assayed at pH 6.0 in the presence of NADH or NADPH Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 29000, SDS-PAGE Escherichia coli
monomer 1 * 31003, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
2,5-diketo-D-gluconate reductase
-
 Escherichia coli
2,5-diketo-D-gluconate-reductase
-
 Escherichia coli
25DKGR-A
-
 Escherichia coli
dkgA
-
 Escherichia coli
dkgB
-
 Escherichia coli
YqhE
-
 Escherichia coli
YqhE reductase
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Escherichia coli