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Literature summary for 1.1.1.346 extracted from

  • Yum, D-Y.; Bae, S-S.; Pan, J-G.
    Purification and characterization of the 2-ketoaldonate reductase from Brevibacterium ketosoreductum ATCC21914 (1998), Biosci. Biotechnol. Biochem., 62, 154-156.
    View publication on PubMed
Search for more literature for 1.1.1.346

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 NADPH at pH 6.4 and 30°C Brevibacterium ketosoreductum
5
-
 2,5-didehydro-D-gluconate at pH 6.4 and 30°C Brevibacterium ketosoreductum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum
72000
-
 gel filtration Brevibacterium ketosoreductum

Organism

Organism UniProt Comment Textmining
Brevibacterium ketosoreductum
-
-
-
Brevibacterium ketosoreductum ATCC 21914
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DEAE Toyopearl column chromatography and phenyl Toyopearl column chromatography, Blue Sepharose column chromatography and Sephacryl S-200 gel filtration Brevibacterium ketosoreductum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
 crude extract, at pH 6.4 and 30°C Brevibacterium ketosoreductum
22.2
-
 after 317fold purification, at pH 6.4 and 30°C Brevibacterium ketosoreductum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,5-didehydro-D-gluconate + NADPH + H+ 100% activity Brevibacterium ketosoreductum 5-dehydro-D-gluconate + NADP+
-
 ?
2-dehydro-D-gluconate + NADPH + H+ 11.8% activity compared to 2,5-didehydro-D-gluconate Brevibacterium ketosoreductum D-gluconate + NADP+
-
 ?
2-dehydro-L-gulonate + NADPH + H+ 10.8% activity compared to 2,5-didehydro-D-gluconate Brevibacterium ketosoreductum L-idonate + NADP+
-
 ?
additional information no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate Brevibacterium ketosoreductum ?
-
 ?
additional information no activity with D-gluconate, D-fructose, L-sorbose, and 5-dehydro-D-gluconate Brevibacterium ketosoreductum ATCC 21914 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 35000, SDS-PAGE Brevibacterium ketosoreductum

Synonyms

Synonyms Comment Organism
25DKG reductase
-
 Brevibacterium ketosoreductum
2KR
-
 Brevibacterium ketosoreductum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Brevibacterium ketosoreductum

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 10 after 2 h incubation at 30°C, the remaining activity is about 35% of the original level at pH 8.4, and no remaining activity is observed below pH 5.1 and 10.0. The enzyme is stable from pH 6.0 to 7.5 Brevibacterium ketosoreductum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Brevibacterium ketosoreductum

pI Value

Organism Comment pI Value Maximum pI Value
Brevibacterium ketosoreductum isoelectric focusing
-
 4.7