Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
4 * 33000, SDS-PAGE | Bradyrhizobium sp. |
130000 | - |
gel filtration | Bradyrhizobium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
- |
- |
Bradyrhizobium japonicum USDA 110 | - |
- |
- |
Bradyrhizobium sp. | - |
- |
- |
Bradyrhizobium sp. BGA-1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Blue-Sepharose CL-6B column chromatography, DEAE-Sephacel gel filtration, and Sephadex G200 gel filtration | Bradyrhizobium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.089 | - |
crude extract, at 37°C and pH 8.3 | Bradyrhizobium sp. |
31.16 | - |
after 350fold purification, at 37°C and pH 8.3 | Bradyrhizobium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | - |
Bradyrhizobium japonicum | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
6-phospho-D-gluconate + NAD+ | - |
Bradyrhizobium sp. | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
6-phospho-D-gluconate + NAD+ | - |
Bradyrhizobium japonicum USDA 110 | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
6-phospho-D-gluconate + NAD+ | - |
Bradyrhizobium sp. BGA-1 | D-ribulose 5-phosphate + CO2 + NADH + H+ | - |
? | |
additional information | no NADP-dependent activity is detected | Bradyrhizobium japonicum | ? | - |
? | |
additional information | no NADP-dependent activity is detected | Bradyrhizobium sp. | ? | - |
? | |
additional information | no NADP-dependent activity is detected | Bradyrhizobium japonicum USDA 110 | ? | - |
? | |
additional information | no NADP-dependent activity is detected | Bradyrhizobium sp. BGA-1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 33000, SDS-PAGE | Bradyrhizobium sp. |
Synonyms | Comment | Organism |
---|---|---|
NAD+-dependent 6-phosphogluconate dehydrogenase (decarboxylating) | - |
Bradyrhizobium japonicum |
NAD+-dependent 6-phosphogluconate dehydrogenase (decarboxylating) | - |
Bradyrhizobium sp. |
NAD-dependent PGDH | - |
Bradyrhizobium japonicum |
NAD-dependent PGDH | - |
Bradyrhizobium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the enzyme is stable after a treatment at 60°C for 30 min | Bradyrhizobium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bradyrhizobium japonicum | |
NAD+ | - |
Bradyrhizobium sp. |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme commits hexoses to the pentose phosphate pathway | Bradyrhizobium japonicum |
metabolism | the enzyme commits hexoses to the pentose phosphate pathway | Bradyrhizobium sp. |