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Literature summary for 1.1.1.342 extracted from

  • Hallis, T.M.; Lei, Y.; Que, N.L.; Liu, H.
    Mechanistic studies of the biosynthesis of paratose: purification and characterization of CDP-paratose synthase (1998), Biochemistry, 37, 4935-4945.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhi

General Stability

General Stability Organism
enzyme is very unstable at low protein concentration Salmonella enterica subsp. enterica serovar Typhi

Inhibitors

Inhibitors Comment Organism Structure
adenosine triphosphate ribose competitive inhibitor with respect to NADPH and either an uncompetitive or a mixed type inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose Salmonella enterica subsp. enterica serovar Typhi
CDP competitive inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and either an uncompetitive or a mixed type inhibitor with respect to NADPH Salmonella enterica subsp. enterica serovar Typhi
CDP-paratose competitive inhibitor against CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and mixed type or uncompetitive inhibitor with respect to NADPH Salmonella enterica subsp. enterica serovar Typhi
NADP+ mixed type or uncompetitive inhibitor with respect to CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose and competitive inhibitor for NADPH Salmonella enterica subsp. enterica serovar Typhi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.026
-
NADPH pH 8.0, 25°C Salmonella enterica subsp. enterica serovar Typhi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
2 * 29000, SDS-PAGE, 2 * 31501, calculated Salmonella enterica subsp. enterica serovar Typhi
31501
-
2 * 29000, SDS-PAGE, 2 * 31501, calculated Salmonella enterica subsp. enterica serovar Typhi
55000
-
gel filtration Salmonella enterica subsp. enterica serovar Typhi

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhi
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
93
-
pH 8.0, 25°C Salmonella enterica subsp. enterica serovar Typhi

Storage Stability

Storage Stability Organism
-80°C, stable for at least 1 year Salmonella enterica subsp. enterica serovar Typhi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CDP-4-dehydro-3,6-dideoxy-alpha-D-glucose + NADPH + H+
-
Salmonella enterica subsp. enterica serovar Typhi CDP-alpha-D-paratose + NADP+ conversion is almost quantitative ?
additional information enzyme catalyzes the stereospecific hydride transfer of the pro-S hydrogen from the C-4' position of the reduced coenzyme to C-4 of the substrate, CDP-3,6-dideoxy-D-glycero-D-glycero-4-hexulose. The overall equilibrium of this catalysis greatly favors the formation of the reduced sugar product and the oxidized coenzyme Salmonella enterica subsp. enterica serovar Typhi ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 29000, SDS-PAGE, 2 * 31501, calculated Salmonella enterica subsp. enterica serovar Typhi

Synonyms

Synonyms Comment Organism
rfbS
-
Salmonella enterica subsp. enterica serovar Typhi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
buffer 0.2 M potassium phosphate Salmonella enterica subsp. enterica serovar Typhi

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is 10fold prefered over NADH Salmonella enterica subsp. enterica serovar Typhi
NADPH NADPH is 10fold prefered over NADH. Enzyme exhibits a high affinity for NADPH with a much smaller dissociation constant of 0.005microM compared to the Km of 0.26 mM Salmonella enterica subsp. enterica serovar Typhi