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Literature summary for 1.1.1.327 extracted from

  • Hofer, M.; Strittmatter, H.; Sieber, V.
    Biocatalytic synthesis of a diketobornane as a building block for bifunctional camphor derivatives (2013), ChemCatChem, 5, 3351-3357.
No PubMed abstract available

Application

Application Comment Organism
synthesis synthetic route for the synthesis of bifunctional camphor derivatives. The combination of the enzymatic P450cam system with 5-exo-hydroxycamphor dehydrogenase (FdeH) allows an efficient synthesis of 2,5-diketobornane, which can be used for further derivatisation Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0279
-
5-exo-hydroxycamphor pH 8.0, 45°C Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida P09347 sequence shows one nucleotide exchange at position 336, at which replacement of a guanine by a cytosine takes place
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-exo-hydroxycamphor + NAD+
-
Pseudomonas putida bornane-2,5-dione + NADH + H+
-
r
bornane-2,5-dione + NADH + H+
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Pseudomonas putida 5-exo-hydroxycamphor + NAD+
-
r
additional information no substrate: camphor Pseudomonas putida ?
-
?

Synonyms

Synonyms Comment Organism
camD
-
Pseudomonas putida
FdeH
-
Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Pseudomonas putida

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60
-
no residual activity Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Pseudomonas putida