Application | Comment | Organism |
---|---|---|
synthesis | synthetic route for the synthesis of bifunctional camphor derivatives. The combination of the enzymatic P450cam system with 5-exo-hydroxycamphor dehydrogenase (FdeH) allows an efficient synthesis of 2,5-diketobornane, which can be used for further derivatisation | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0279 | - |
5-exo-hydroxycamphor | pH 8.0, 45°C | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | P09347 | sequence shows one nucleotide exchange at position 336, at which replacement of a guanine by a cytosine takes place | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-exo-hydroxycamphor + NAD+ | - |
Pseudomonas putida | bornane-2,5-dione + NADH + H+ | - |
r | |
bornane-2,5-dione + NADH + H+ | - |
Pseudomonas putida | 5-exo-hydroxycamphor + NAD+ | - |
r | |
additional information | no substrate: camphor | Pseudomonas putida | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
camD | - |
Pseudomonas putida |
FdeH | - |
Pseudomonas putida |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pseudomonas putida |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
no residual activity | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas putida |