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Literature summary for 1.1.1.318 extracted from
- The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida represent two distinct protein lineages (2008), Plant J., 54, 362-374.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis | Petunia x hybrida |
| DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, expression of nontagged and of His-tagged CbEGS1 and CbEGS2 in Escherichia coli | Clarkia breweri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| F84V/I87Y | site-directed mutagenesis, the CbEGS1 mutant plants show increased isoeugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| F86V/I89Y | site-directed mutagenesis, the CbEGS2 mutant plants show increased isoeugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Clarkia breweri |
| Q86V/L89Y | site-directed mutagenesis, the mutant plants show increased isoeugenol production and an altered eugenol/isoeugenol ratio compared to the wild-type enzyme | Petunia x hybrida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0933 | - |
coniferyl acetate | CbEGS1, pH 6.5, 28°C | Clarkia breweri |  |
| 0.2453 | - |
coniferyl acetate | PhEGS1, pH 6.5, 28°C | Petunia x hybrida | |
| 0.3105 | - |
coniferyl acetate | CbEGS2, pH 6.5, 28°C | Clarkia breweri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36000 | - |
x * 38000, recombinant nontagged CbEGS2, SDS-PAGE, x * 36000, recombinant nontagged CbEGS1, SDS-PAGE | Clarkia breweri |
| 38000 | - |
x * 38000, recombinant nontagged CbEGS2, SDS-PAGE, x * 36000, recombinant nontagged CbEGS1, SDS-PAGE | Clarkia breweri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | Ocimum basilicum | - |
eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | Clarkia breweri | - |
eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | Petunia x hybrida | PhEGS1 converts coniferyl acetate to eugenol | eugenol + acetate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clarkia breweri | - |
- |
- |
| Ocimum basilicum | - |
- |
- |
| Petunia x hybrida | - |
cv. Mitchell | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native EGS 118fold from petals by alternating steps of anion exchange and hydrophobic interaction chromatography, co-purification with isoeugenol synthase, EC 1.1.1.319. Recombinant His-tagged CbEGS1 and CbEGS2 from Escherichia coli by nickel affinity chromatography | Clarkia breweri |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Ocimum basilicum | - |
| flower | PhEGS1 is expressed specifically in the scent-producing parts of the flowers, limbs and tube, but not in other parts of the flowers nor in leaves | Petunia x hybrida | - |
| flower | the highest specific activity levels of eugenol synthase are found in the stamens, followed closely by the pistil and petals | Clarkia breweri | - |
| petal | - |
Clarkia breweri | - |
| pistil | - |
Clarkia breweri | - |
| stamen | - |
Clarkia breweri | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.98 | - |
purified native, wild-type CbEGS, pH 6.5, 28°C | Clarkia breweri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | - |
Ocimum basilicum | eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | - |
Petunia x hybrida | eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | - |
Clarkia breweri | eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | PhEGS1 converts coniferyl acetate to eugenol | Petunia x hybrida | eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | CbEGS1 is specific for isoeugenol production | Clarkia breweri | eugenol + acetate + NADP+ | - |
? | |
| coniferyl acetate + NADPH + H+ | ObEGS1 uses a quinone methide intermediate-based mechanism to generate an intermediate to which the reductive transfer of the reducing hydride can then be easily accomplished. the enzyme acts on the substrate via a push-pull mechanism, removing the proton of the para hydroxyl group and promoting the cleavage of the acetyl group. In the resultant quinone-methide intermediate, the C7 atom serves as the acceptor of the hydride ion from NADPH | Ocimum basilicum | eugenol + acetate + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, recombinant nontagged CbEGS2, SDS-PAGE, x * 36000, recombinant nontagged CbEGS1, SDS-PAGE | Clarkia breweri |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CbEGS1 | - |
Clarkia breweri |
| CbEGS2 | - |
Clarkia breweri |
| EGS | - |
Ocimum basilicum |
| EGS | - |
Petunia x hybrida |
| EGS | - |
Clarkia breweri |
| ObEGS1 | - |
Ocimum basilicum |
| PhEGS1 | - |
Petunia x hybrida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Petunia x hybrida |
| 28 | - |
assay at | Clarkia breweri |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
coniferyl acetate | CbEGS2, pH 6.5, 28°C | Clarkia breweri | |
| 0.26 | - |
coniferyl acetate | CbEGS1, pH 6.5, 28°C | Clarkia breweri | |
| 0.6 | - |
coniferyl acetate | PhEGS1, pH 6.5, 28°C | Petunia x hybrida | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Petunia x hybrida |
| 6.5 | - |
assay at | Clarkia breweri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Petunia x hybrida | |
| NADPH | - |
Clarkia breweri | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Clarkia breweri flowers emit a mixture of eugenol and isoeugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain | Clarkia breweri |
| physiological function | plants synthesize the volatile phenylpropene compounds eugenol and isoeugenol to serve in defense against herbivores and pathogens and to attract pollinators. Petunia hybrida flowers emit mostly isoeugenol with small amounts of eugenol. Eugenol and isoeugenol differ in the position of the double bond in the propene side chain | Petunia x hybrida |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
coniferyl acetate | CbEGS2, pH 6.5, 28°C | Clarkia breweri | |
| 2.4 | - |
coniferyl acetate | PhEGS1, pH 6.5, 28°C | Petunia x hybrida | |
| 2.8 | - |
coniferyl acetate | CbEGS1, pH 6.5, 28°C | Clarkia breweri | |
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