Cloned (Comment) | Organism |
---|---|
- |
Pseudomonas straminea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
NADP+ | 25°C | Pseudomonas straminea | |
0.075 | - |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde | 25°C | Pseudomonas straminea | |
0.314 | - |
NADP+ | 25°C | Pseudomonas straminea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Pseudomonas straminea |
67000 | - |
gel filtration | Pseudomonas straminea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas straminea | - |
NGJ1 | - |
Pseudomonas straminea NGJ1 | - |
NGJ1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Pseudomonas straminea |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
549 | - |
25°C | Pseudomonas straminea |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ | NAD+ is less effective than NADP+ | Pseudomonas straminea | 4-carboxy-2-hydroxy-cis,cis-muconate + NADH | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ | NAD+ is less effective than NADP+ | Pseudomonas straminea NGJ1 | 4-carboxy-2-hydroxy-cis,cis-muconate + NADH | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ | NAD+ is less effective than NADP+ | Pseudomonas straminea | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH | - |
? | |
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ | NAD+ is less effective than NADP+ | Pseudomonas straminea NGJ1 | 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Pseudomonas straminea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas straminea |