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Literature summary for 1.1.1.312 extracted from

  • Maruyama, K.; Shibayama, T.; Ichikawa, A.; Sakou, Y.; Yamada, S.; Sugisaki, H.
    Cloning and characterization of the genes encoding enzymes for the protocatechuate meta-degradation pathway of Pseudomonas ochraceae NGJ1 (2004), Biosci. Biotechnol. Biochem., 68, 1434-1441.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Pseudomonas straminea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0061
-
NADP+ 25°C Pseudomonas straminea
0.075
-
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde 25°C Pseudomonas straminea
0.314
-
NADP+ 25°C Pseudomonas straminea

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
2 * 35000, SDS-PAGE Pseudomonas straminea
67000
-
gel filtration Pseudomonas straminea

Organism

Organism UniProt Comment Textmining
Pseudomonas straminea
-
NGJ1
-
Pseudomonas straminea NGJ1
-
NGJ1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme expressed in Escherichia coli Pseudomonas straminea

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
549
-
25°C Pseudomonas straminea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ NAD+ is less effective than NADP+ Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NAD+ NAD+ is less effective than NADP+ Pseudomonas straminea NGJ1 4-carboxy-2-hydroxy-cis,cis-muconate + NADH
-
?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ NAD+ is less effective than NADP+ Pseudomonas straminea 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH
-
?
4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde + NADP+ NAD+ is less effective than NADP+ Pseudomonas straminea NGJ1 4-carboxy-2-hydroxy-cis,cis-muconate + NADPH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Pseudomonas straminea

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Pseudomonas straminea