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Literature summary for 1.1.1.310 extracted from

  • Denger, K.; Cook, A.M.
    Racemase activity effected by two dehydrogenases in sulfolactate degradation by Chromohalobacter salexigens: purification of (S)-sulfolactate dehydrogenase (2010), Microbiology, 156, 967-974.
    View publication on PubMed
Search for more literature for 1.1.1.310

General Stability

General Stability Organism
at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity Chromohalobacter israelensis

Inhibitors

Inhibitors Comment Organism Structure
additional information SlcC is not inhibited by pyruvate or oxaloacetate (5 mM), (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate Chromohalobacter israelensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 NADH apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter israelensis
0.14
-
 3-sulfopyruvate apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter israelensis
2
-
 NAD+ apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter israelensis
7
-
 (2S)-3-sulfolactate apparent value, in 25 mM Tris buffer, pH 9.0 and 25°C Chromohalobacter israelensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
 2 * 34000, SDS-PAGE Chromohalobacter israelensis
61000
-
 native enzyme, gel filtration Chromohalobacter israelensis

Organism

Organism UniProt Comment Textmining
Chromohalobacter israelensis
-
-
-
Chromohalobacter israelensis DSM 3043
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Mono Q column chromatography, phenyl Superose gel filtration, Superose 12 gel filtration Chromohalobacter israelensis

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:acetate-grown cell
-
 Chromohalobacter israelensis
-
culture condition:sulfolactate-grown cell
-
 Chromohalobacter israelensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 crude extracts of acetate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter israelensis
7.4
-
 crude extracts of sulfolactate-grown cells, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter israelensis
675
-
 enzyme from sulfolactate-grown cells after 91fold purification, using 3-sulfopyruvate as substrate, at pH 9.0 and 25°C Chromohalobacter israelensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S)-3-sulfolactate + NAD+ the specific activity is only about 5% of that in the reverse direction Chromohalobacter israelensis 3-sulfopyruvate + NADH + H+
-
 r
(2S)-3-sulfolactate + NAD+ the specific activity is only about 5% of that in the reverse direction Chromohalobacter israelensis DSM 3043 3-sulfopyruvate + NADH + H+
-
 r
3-sulfopyruvate + NADH + H+
-
 Chromohalobacter israelensis (2S)-3-sulfolactate + NAD+
-
 r
3-sulfopyruvate + NADH + H+
-
 Chromohalobacter israelensis DSM 3043 (2S)-3-sulfolactate + NAD+
-
 r
additional information neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate Chromohalobacter israelensis ?
-
 ?
additional information neither pyruvate, oxaloacetate, (S)-3-phosphoglycerate, (R)-malate, (R)-lactate, (S)-malate or (S)-lactate is a substrate Chromohalobacter israelensis DSM 3043 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 34000, SDS-PAGE Chromohalobacter israelensis

Synonyms

Synonyms Comment Organism
(S)-sulfolactate oxidoreductase
-
 Chromohalobacter israelensis
SlcC
-
 Chromohalobacter israelensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 at 4°C, SlcC remains stable for several weeks and can be frozen and thawed without loss of activity Chromohalobacter israelensis