Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | BDH1 is abundant in cells grown on D-fructose, D-glucose, maltose, oxaloacetate, 2-oxoglutarate, L-alanine, L-arginine, L-methionine, poly-3-hydroxybutyrate, and nutrient broth, and poor in cells grown on pyruvate, gluconate, fumarate, L-malate, propionate, and n-butyrate | Ralstonia pickettii | |
additional information | BDH2 is abundant in cells grown on acetate, ethanol, D-arabinose, D-ribose, oxaloacetate, 2-oxoglutarate, malaete, L-histidine, L-alanine, L-arginine, L-methionine, and poly-3-hydroxybutyrate, and scant in cells grown on pyruvate, n-butyrate, and oleate | Ralstonia pickettii | |
additional information | BDH3 is produced only in cells grown on 3-hydroxybutyrate or poly-3-hydroxybutyrate as a carbon source. Expression of bdh3 may be coordinately regulated with a gene encoding putative 3-hydroxybutyrate permease | Ralstonia pickettii |
Cloned (Comment) | Organism |
---|---|
BDH3 expressed in Escherichia coli BLR (DE3)/pLysS harboring pETT13 | Ralstonia pickettii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the bdh1 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH1 causes a decline in the capacity to neutralize the stress | Ralstonia pickettii |
additional information | the bdh2 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH2 causes a decline in the capacity to neutralize the stress | Ralstonia pickettii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diamide | - |
Ralstonia pickettii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
NAD+ | - |
Ralstonia pickettii | |
0.1 | - |
NAD+ | - |
Ralstonia pickettii | |
0.24 | - |
NADH | - |
Ralstonia pickettii | |
0.25 | - |
acetoacetate | - |
Ralstonia pickettii | |
0.31 | - |
NAD+ | - |
Ralstonia pickettii | |
0.32 | - |
NADH | - |
Ralstonia pickettii | |
0.34 | - |
D-3-hydroxybutyrate | - |
Ralstonia pickettii | |
0.49 | - |
D-3-hydroxybutyrate | - |
Ralstonia pickettii | |
0.5 | - |
D-3-hydroxybutyrate | - |
Ralstonia pickettii | |
1.1 | - |
NADH | - |
Ralstonia pickettii | |
1.1 | - |
acetoacetate | - |
Ralstonia pickettii | |
1.3 | - |
acetoacetate | - |
Ralstonia pickettii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE, BDH3 | Ralstonia pickettii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia pickettii | A8R3J3 | T1 | - |
Ralstonia pickettii | Q2PEN2 | T1 | - |
Ralstonia pickettii | Q2PEN3 | T1 | - |
Ralstonia pickettii T1 | A8R3J3 | T1 | - |
Ralstonia pickettii T1 | Q2PEN2 | T1 | - |
Ralstonia pickettii T1 | Q2PEN3 | T1 | - |
Purification (Comment) | Organism |
---|---|
BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography | Ralstonia pickettii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1200 | - |
purified BDH3 | Ralstonia pickettii |
Storage Stability | Organism |
---|---|
-20°C, 20 mM phosphate buffer, pH 7.0, 20% glycerol | Ralstonia pickettii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-3-hydroxybutyrate + NAD+ | BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate | Ralstonia pickettii | acetoacetate + NADH + H+ | - |
r | |
D-3-hydroxybutyrate + NAD+ | BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate | Ralstonia pickettii | acetoacetate + NADH + H+ | - |
r | |
D-3-hydroxybutyrate + NAD+ | BDH3 is specialized to utilize 3-hydroxybutyrate | Ralstonia pickettii | acetoacetate + NADH + H+ | - |
r | |
D-3-hydroxybutyrate + NAD+ | BDH3 is specialized to utilize 3-hydroxybutyrate | Ralstonia pickettii T1 | acetoacetate + NADH + H+ | - |
r | |
D-3-hydroxybutyrate + NAD+ | BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate | Ralstonia pickettii T1 | acetoacetate + NADH + H+ | - |
r | |
D-3-hydroxybutyrate + NAD+ | BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate | Ralstonia pickettii T1 | acetoacetate + NADH + H+ | - |
r | |
poly-3-hydroxybutyrate + NAD+ | - |
Ralstonia pickettii | acetoacetate + NADH + H+ | - |
r | |
poly-3-hydroxybutyrate + NAD+ | - |
Ralstonia pickettii T1 | acetoacetate + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE, BDH3 | Ralstonia pickettii |
Synonyms | Comment | Organism |
---|---|---|
BDH1 | - |
Ralstonia pickettii |
BDH2 | - |
Ralstonia pickettii |
BDH3 | - |
Ralstonia pickettii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58 | - |
NADH | - |
Ralstonia pickettii | |
87 | - |
NAD+ | - |
Ralstonia pickettii | |
100 | - |
acetoacetate | - |
Ralstonia pickettii | |
110 | - |
acetoacetate | - |
Ralstonia pickettii | |
150 | - |
acetoacetate | - |
Ralstonia pickettii | |
180 | - |
D-3-hydroxybutyrate | - |
Ralstonia pickettii | |
280 | - |
NADH | - |
Ralstonia pickettii | |
310 | - |
D-3-hydroxybutyrate | - |
Ralstonia pickettii | |
340 | - |
NAD+ | - |
Ralstonia pickettii | |
360 | - |
NAD+ | - |
Ralstonia pickettii | |
500 | - |
D-3-hydroxybutyrate | - |
Ralstonia pickettii | |
740 | - |
NADH | - |
Ralstonia pickettii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | optimal pH of BDH3 is 8.5 in the oxidation reaction and 6.5 in the reduction reaction | Ralstonia pickettii |