Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.30 extracted from

  • Takanashi, M.; Shiraki, M.; Saito, T.
    Characterization of a novel 3-hydroxybutyrate dehydrogenase from Ralstonia pickettii T1 (2009), Antonie van Leeuwenhoek, 95, 249-262.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information BDH1 is abundant in cells grown on D-fructose, D-glucose, maltose, oxaloacetate, 2-oxoglutarate, L-alanine, L-arginine, L-methionine, poly-3-hydroxybutyrate, and nutrient broth, and poor in cells grown on pyruvate, gluconate, fumarate, L-malate, propionate, and n-butyrate Ralstonia pickettii
additional information BDH2 is abundant in cells grown on acetate, ethanol, D-arabinose, D-ribose, oxaloacetate, 2-oxoglutarate, malaete, L-histidine, L-alanine, L-arginine, L-methionine, and poly-3-hydroxybutyrate, and scant in cells grown on pyruvate, n-butyrate, and oleate Ralstonia pickettii
additional information BDH3 is produced only in cells grown on 3-hydroxybutyrate or poly-3-hydroxybutyrate as a carbon source. Expression of bdh3 may be coordinately regulated with a gene encoding putative 3-hydroxybutyrate permease Ralstonia pickettii

Cloned(Commentary)

Cloned (Comment) Organism
BDH3 expressed in Escherichia coli BLR (DE3)/pLysS harboring pETT13 Ralstonia pickettii

Protein Variants

Protein Variants Comment Organism
additional information the bdh1 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH1 causes a decline in the capacity to neutralize the stress Ralstonia pickettii
additional information the bdh2 mutant lags behind the wild-type in growth rates when the cells are cultured with 3-hydroxybutyrate, citrate, succinate, or nutrient broth. A test of sensitivity to diamide as an oxidative stress reveals that the lack of BDH2 causes a decline in the capacity to neutralize the stress Ralstonia pickettii

Inhibitors

Inhibitors Comment Organism Structure
Diamide
-
Ralstonia pickettii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.064
-
NAD+
-
Ralstonia pickettii
0.1
-
NAD+
-
Ralstonia pickettii
0.24
-
NADH
-
Ralstonia pickettii
0.25
-
acetoacetate
-
Ralstonia pickettii
0.31
-
NAD+
-
Ralstonia pickettii
0.32
-
NADH
-
Ralstonia pickettii
0.34
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
0.49
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
0.5
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
1.1
-
NADH
-
Ralstonia pickettii
1.1
-
acetoacetate
-
Ralstonia pickettii
1.3
-
acetoacetate
-
Ralstonia pickettii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
x * 28000, SDS-PAGE, BDH3 Ralstonia pickettii

Organism

Organism UniProt Comment Textmining
Ralstonia pickettii A8R3J3 T1
-
Ralstonia pickettii Q2PEN2 T1
-
Ralstonia pickettii Q2PEN3 T1
-
Ralstonia pickettii T1 A8R3J3 T1
-
Ralstonia pickettii T1 Q2PEN2 T1
-
Ralstonia pickettii T1 Q2PEN3 T1
-

Purification (Commentary)

Purification (Comment) Organism
BDH3 partially purified from bdh2 mutant by three steps of column chromatography. Gene product of bdh3 expressed in Escherichia coli purified with 100% yield by two steps of column chromatography Ralstonia pickettii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1200
-
purified BDH3 Ralstonia pickettii

Storage Stability

Storage Stability Organism
-20°C, 20 mM phosphate buffer, pH 7.0, 20% glycerol Ralstonia pickettii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-3-hydroxybutyrate + NAD+ BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii acetoacetate + NADH + H+
-
r
D-3-hydroxybutyrate + NAD+ BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii acetoacetate + NADH + H+
-
r
D-3-hydroxybutyrate + NAD+ BDH3 is specialized to utilize 3-hydroxybutyrate Ralstonia pickettii acetoacetate + NADH + H+
-
r
D-3-hydroxybutyrate + NAD+ BDH3 is specialized to utilize 3-hydroxybutyrate Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r
D-3-hydroxybutyrate + NAD+ BDH2 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r
D-3-hydroxybutyrate + NAD+ BDH1 is needed to regulate the cytoplasmic redox state as well as to utilize 3-hydroxybutyrate Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r
poly-3-hydroxybutyrate + NAD+
-
Ralstonia pickettii acetoacetate + NADH + H+
-
r
poly-3-hydroxybutyrate + NAD+
-
Ralstonia pickettii T1 acetoacetate + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 28000, SDS-PAGE, BDH3 Ralstonia pickettii

Synonyms

Synonyms Comment Organism
BDH1
-
Ralstonia pickettii
BDH2
-
Ralstonia pickettii
BDH3
-
Ralstonia pickettii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
58
-
NADH
-
Ralstonia pickettii
87
-
NAD+
-
Ralstonia pickettii
100
-
acetoacetate
-
Ralstonia pickettii
110
-
acetoacetate
-
Ralstonia pickettii
150
-
acetoacetate
-
Ralstonia pickettii
180
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
280
-
NADH
-
Ralstonia pickettii
310
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
340
-
NAD+
-
Ralstonia pickettii
360
-
NAD+
-
Ralstonia pickettii
500
-
D-3-hydroxybutyrate
-
Ralstonia pickettii
740
-
NADH
-
Ralstonia pickettii

pH Range

pH Minimum pH Maximum Comment Organism
6.5 8.5 optimal pH of BDH3 is 8.5 in the oxidation reaction and 6.5 in the reduction reaction Ralstonia pickettii