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Literature summary for 1.1.1.30 extracted from
- D-3-hydroxybutyrate dehydrogenase from Pseudomonas fragi: molecular cloning of the enzyme gene and crystal structure of the enzyme (2006), J. Mol. Biol., 355, 722-733.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| efficiently expressed in Escherichia coli cells harboring pHBDH11 | Pseudomonas fragi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor-diffusion method, ligand-free enzyme and enzyme-NAD+ complex, 2.0 A resolution | Pseudomonas fragi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cacodylate | competitive | Pseudomonas fragi |  |
| dimethyl malonate | - |
Pseudomonas fragi | |
| DL-2-hydroxybutyrate | - |
Pseudomonas fragi | |
| DL-lactate | - |
Pseudomonas fragi | |
| malonate | - |
Pseudomonas fragi | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
NAD+ | - |
Pseudomonas fragi | |
| 1.2 | - |
(R)-3-hydroxybutanoate | - |
Pseudomonas fragi | |
| 7.4 | - |
NADP+ | - |
Pseudomonas fragi | |
| 388 | - |
L-threonine | - |
Pseudomonas fragi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, SDS-PAGE | Pseudomonas fragi |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fragi | Q5KST5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Pseudomonas fragi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-3-hydroxybutanoate + NAD+ | - |
Pseudomonas fragi | acetoacetate + NADH | - |
? | |
| (R)-3-hydroxybutanoate + NADP+ | activity with NADP+ is 2% of the activity with NAD+ | Pseudomonas fragi | acetoacetate + NADPH | - |
? | |
| L-threonine + NAD+ | - |
Pseudomonas fragi | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, SDS-PAGE | Pseudomonas fragi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reduction | Pseudomonas fragi |
| 8.5 | - |
dehydrogenation | Pseudomonas fragi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pseudomonas fragi | |
| NADP+ | 2% of the activity with NAD+ | Pseudomonas fragi | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.81 | - |
DL-2-hydroxybutyrate | - |
Pseudomonas fragi | |
| 0.9 | - |
DL-lactate | - |
Pseudomonas fragi | |
| 3.8 | - |
malonate | - |
Pseudomonas fragi | |
| 5.6 | - |
cacodylate | - |
Pseudomonas fragi | |
| 80 | - |
dimethyl malonate | - |
Pseudomonas fragi | |
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