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Literature summary for 1.1.1.30 extracted from

  • Feller, C.; Guenther, R.; Hofmann, H.J.; Grunow, M.
    Molecular basis of substrate recognition in D-3-hydroxybutyrate dehydrogenase from Pseudomonas putida (2006), ChemBioChem, 7, 1410-1418.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
H141A kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 12.9fold lower than wild-type enzyme Pseudomonas putida
K149A inactive mutant enzyme Pseudomonas putida
K149R kcat/KM for (R)-3-hydroxybutanoate is 184.2fold lower than wild-type value, kcat/Km for NAD+ is 7.2fold lower than wild-type enzyme Pseudomonas putida
Q193A kcat/KM for (R)-3-hydroxybutanoate is 307fold lower than wild-type value, kcat/Km for NAD+ is 6.2fold lower than wild-type enzyme Pseudomonas putida
Q91A kcat/KM for (R)-3-hydroxybutanoate is 83.7fold lower than wild-type value, kcat/Km for NAD+ is 2.8fold lower than wild-type enzyme Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
NAD+ 23°C, pH 8.0, wild-type enzyme Pseudomonas putida
0.27
-
NAD+ 23°C, pH 8.0, mutant enzyme Q193A Pseudomonas putida
0.28
-
NAD+ 23°C, pH 8.0, mutant enzyme H141A Pseudomonas putida
0.38
-
NAD+ 23°C, pH 8.0, mutant enzyme K149R Pseudomonas putida
0.51
-
NAD+ 23°C, pH 8.0, mutant enzyme Q91A Pseudomonas putida
1.5
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, wild-type enzyme Pseudomonas putida
35
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme H141A Pseudomonas putida
78
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme K149R Pseudomonas putida
96
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q193A Pseudomonas putida
131
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q91A Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida Q9AE70
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
Pseudomonas putida acetoacetate + NADH + H+
-
r

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
167
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme H141A Pseudomonas putida
333
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q193A Pseudomonas putida
404
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme K149R Pseudomonas putida
1382
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, wild-type enzyme Pseudomonas putida
1390
-
(R)-3-hydroxybutanoate 23°C, pH 8.0, mutant enzyme Q91A Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida