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Literature summary for 1.1.1.30 extracted from

  • Scawen, M.D.; Darbyshire, J.; Harvey, M.J.; Atkinson, T.
    The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices (1982), Biochem. J., 203, 699-705.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine
-
Cereibacter sphaeroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
alpha4, 4 * 23000, SDS-PAGE Cereibacter sphaeroides
89000
-
gel filtration Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+ Cereibacter sphaeroides
-
acetoacetate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Cereibacter sphaeroides
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography on two triazine dyes Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.2
-
-
Cereibacter sphaeroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
Cereibacter sphaeroides acetoacetate + NADH
-
r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 23000, SDS-PAGE Cereibacter sphaeroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Cereibacter sphaeroides