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Literature summary for 1.1.1.30 extracted from

  • Matyskova I.; Kovar, J.; Racek, P.
    Purification and properties of D-3-hydroxybutyrate dehydrogenase from Paracoccus denitrificans (1985), Biochim. Biophys. Acta, 839, 300-307.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
phosphatidylcholine
-
Paracoccus denitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
alpha4, 4 * 32000, SDS-PAGE Paracoccus denitrificans
132000
-
gel filtration Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+ Paracoccus denitrificans
-
acetoacetate + NADH
-
r

Organism

Organism UniProt Comment Textmining
Paracoccus denitrificans
-
enzyme shows hysteresis, lag phase in progress curve
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-cellulose, Matrex gel blue A, Sephadex G-200, chromatofocusing Paracoccus denitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97
-
-
Paracoccus denitrificans

Storage Stability

Storage Stability Organism
-20°C, several months, 0%, 4°C, lyophilized, 2 month, 0% inactivation, 4°C, 0.1 M sodium phosphate buffer pH 7-8, 5 days, 50% inactivation, stability increases in the presence of 1-10 mM EDTA Paracoccus denitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-3-hydroxybutanoate + NAD+
-
Paracoccus denitrificans acetoacetate + NADH
-
r

Subunits

Subunits Comment Organism
tetramer alpha4, 4 * 32000, SDS-PAGE Paracoccus denitrificans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Paracoccus denitrificans