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Literature summary for 1.1.1.298 extracted from

  • Kockelkorn, D.; Fuchs, G.
    Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: Enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales (2009), J. Bacteriol., 191, 6352-6362.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.298

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Rosetta 2(DE3) Metallosphaera sedula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.07
-
 NADPH pH 7.4, 65°C Metallosphaera sedula
0.07
-
 malonate semialdehyde pH 7.4, 65°C Metallosphaera sedula

Metals/Ions

Metals/Ions Comment Organism Structure
additional information addition of Zn2+ (0-0.04 mM) or Mg2+or Mn2+(0-5 mM) to the enzyme assay mixture neither stimulates nor inactivates the enzyme Metallosphaera sedula

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 3 * 33000, SDS-PAGE Metallosphaera sedula
105000
-
 gel filtration Metallosphaera sedula

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
malonate semialdehyde + NADPH + H+ Metallosphaera sedula the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 3-hydroxypropanoate + NADP+
-
 ?
malonate semialdehyde + NADPH + H+ Metallosphaera sedula DSM 5348 the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea 3-hydroxypropanoate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Metallosphaera sedula A4YI81
-
-
Metallosphaera sedula DSM 5348 A4YI81
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Metallosphaera sedula

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:autotrophically-grown cell low activity in heterotrophically-grown cell Metallosphaera sedula
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
200
-
 pH 7.4, 65°C, heterologously expressed enzyme Metallosphaera sedula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonate semialdehyde + NADH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula 3-hydroxypropanoate + NAD+
-
 ?
malonate semialdehyde + NADH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula DSM 5348 3-hydroxypropanoate + NAD+
-
 ?
malonate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula 3-hydroxypropanoate + NADP+
-
 ?
malonate semialdehyde + NADPH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula 3-hydroxypropanoate + NADP+
-
 ?
malonate semialdehyde + NADPH + H+ the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula DSM 5348 3-hydroxypropanoate + NADP+
-
 ?
malonate semialdehyde + NADPH + H+ NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula DSM 5348 3-hydroxypropanoate + NADP+
-
 ?
additional information succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula ?
-
 ?
additional information succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde do not serve as a substrate Metallosphaera sedula DSM 5348 ?
-
 ?

Subunits

Subunits Comment Organism
homotrimer 3 * 33000, SDS-PAGE Metallosphaera sedula

Synonyms

Synonyms Comment Organism
malonic semialdehyde reductase
-
 Metallosphaera sedula
Msed_1993 locus name Metallosphaera sedula

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 assay at Metallosphaera sedula
75
-
-
 Metallosphaera sedula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
115
-
 NADPH pH 7.4, 65°C Metallosphaera sedula
115
-
 malonate semialdehyde pH 7.4, 65°C Metallosphaera sedula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.7
-
 optimum pH at 65°C Metallosphaera sedula
7.4
-
 asay at Metallosphaera sedula

Cofactor

Cofactor Comment Organism Structure
NADH NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula
NADPH NADH can partially substitute (20% activity) for NADPH Metallosphaera sedula

Expression

Organism Comment Expression
Metallosphaera sedula the specific activity of this conversion in autotrophically grown cells is 1.8 microM/min * mg protein, whereas in heterotrophically grown cells the specific activity is 0.35 microM/min * mg protein up

General Information

General Information Comment Organism
metabolism the enzyme participates in the 3-hydroxypropionate/4-hydroxybutyrate cycle, an autotrophic CO2 fixation pathway found in some thermoacidophilic archaea Metallosphaera sedula