Cloned (Comment) | Organism |
---|---|
coding region of chlorophyll b reductase (NOL) lacking its transit peptide amplified and cloned into pET-30a(+) at NspV and HindIII sites, expressed in Escherichia coli Rosetta DE3 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | Arabidopsis nol/nyc1 double mutant, when the genes for chlorophyll b reductases NOL and NYC1 are disrupted, chlorophyll b and photosystem II are not degraded during senescence, whereas other pigment complexes completely disappear | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | chlorophyll b reductase decreases during senescence and finally disappears after 8-day-dark incubation | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, sonication, on a nickel column and by ultrafiltration | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
4°C, 24 hours | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chlorophyll b + NADPH | when purified trimeric photosystem II is incubated with recombinant chlorophyll b reductase (NOL), conversion of chlorophyll b in photosystem II to 7-hydroxymethyl chlorophyll a. Chlorophyll b reductase catalyzes the initial step of photosystem II degradation | Arabidopsis thaliana | 7-hydroxymethyl chlorophyll a + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
chlorophyll b reductase | - |
Arabidopsis thaliana |
NOL | - |
Arabidopsis thaliana |
NYC1 | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Arabidopsis thaliana |