Cloned (Comment) | Organism |
---|---|
five putative SDH-encoding genes amplified and cloned into either a modified pET28a vector or the p15TVLIC vector, a ligation-independent vector due to incompatible cloning sites between the insert and pET28.3. Expressed in either the Escherichia coli strain BL21 gold (p15TV-LIC constructs) or the Escherichia coli BL21CodonPlus (pET28.3constructs) | Pseudomonas putida KT2440 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.572 | - |
NAD+ | in the presence of shikimate | Pseudomonas putida KT2440 | |
0.783 | - |
quinate | in the presence of 2 mM NAD+ | Pseudomonas putida KT2440 | |
1.083 | - |
NAD+ | in the presence of quinate | Pseudomonas putida KT2440 | |
4.2 | - |
shikimate | in the presence of 2 mM NAD+ | Pseudomonas putida KT2440 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida KT2440 | Q88GF6 | - |
- |
Pseudomonas putida KT2440 | Q88JP1 | - |
- |
Pseudomonas putida KT2440 | Q88K85 | - |
- |
Purification (Comment) | Organism |
---|---|
by nickel-nitrilotriacetic acid affinity chromatography | Pseudomonas putida KT2440 |
Storage Stability | Organism |
---|---|
4°C, 10 mM Tris-HCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol | Pseudomonas putida KT2440 |
4°C, 10 mM TrisHCl buffer, pH 7.5, 500 mM NaCl, 5% glycerol | Pseudomonas putida KT2440 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-quinate + NAD+ | - |
Pseudomonas putida KT2440 | 3-dehydroquinate + NADH + H+ | - |
r | |
additional information | broad extent to which the SDH enzyme superfamily has diversified. 5 evolutionarily distinct SDH homologs in the genome of the common soil-inhabiting bacterium, Pseudomonas putida KT2440 | Pseudomonas putida KT2440 | ? | - |
? | |
quinate + NAD(P)+ | - |
Pseudomonas putida KT2440 | 3-dehydroquinate + NAD(P)H + H+ | - |
? | |
shikimate + NAD(P)+ | - |
Pseudomonas putida KT2440 | 3-dehydroshikimate + NAD(P)H + H+ | - |
? | |
shikimate + NAD+ | - |
Pseudomonas putida KT2440 | 3-dehydroshikimate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
rifI | - |
Pseudomonas putida KT2440 |
SDH | - |
Pseudomonas putida KT2440 |
sdhL | - |
Pseudomonas putida KT2440 |
YdiB | - |
Pseudomonas putida KT2440 |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
37.7 | - |
quinate | in the presence of 2 mM NAD+ | Pseudomonas putida KT2440 | |
55.7 | - |
shikimate | in the presence of 2 mM NAD+ | Pseudomonas putida KT2440 | |
55.7 | - |
NAD+ | in the presence of quinate | Pseudomonas putida KT2440 | |
61.1 | - |
NAD+ | in the presence of shikimate | Pseudomonas putida KT2440 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Pseudomonas putida KT2440 |