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Literature summary for 1.1.1.282 extracted from

  • Lindner, H.A.; Nadeau, G.; Matte, A.; Michel, G.; Menard, R.; Cygler, M.
    Site-directed mutagenesis of the active site region in the quinate/shikimate 5-dehydrogenase YdiB of Escherichia coli (2005), J. Biol. Chem., 280, 7162-7169.
    View publication on PubMed

Application

Application Comment Organism
agriculture development of novel herbicides Escherichia coli
medicine development of novel antimicrobial agents Escherichia coli

Protein Variants

Protein Variants Comment Organism
D107A site-directed mutagenesis Escherichia coli
K71G site-directed mutagenesis Escherichia coli
N92A site-directed mutagenesis Escherichia coli
Q262A site-directed mutagenesis Escherichia coli
S22A site-directed mutagenesis Escherichia coli
S67A site-directed mutagenesis, increased activity compared to wild type enzyme Escherichia coli
T106A site-directed mutagenesis Escherichia coli
Y39F site-directed mutagenesis Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
NAD+ mutant K71A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0017
-
shikimate mutant S67A, 20°C, pH 9.0 Escherichia coli
0.002
-
NAD+ mutant K71A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.0028
-
shikimate mutant Q262A, 20°C, pH 9.0 Escherichia coli
0.0029
-
shikimate wild type enzyme, 20°C, pH 9.0 Escherichia coli
0.0032
-
shikimate mutant S22A, 20°C, pH 9.0 Escherichia coli
0.0032
-
shikimate mutant Y39F, 20°C, pH 9.0 Escherichia coli
0.0054
-
L-quinate mutant Y39F, 20°C, pH 9.0 Escherichia coli
0.0057
-
L-quinate mutant S22A, 20°C, pH 9.0 Escherichia coli
0.0068
-
NAD+ mutant D107A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0091
-
L-quinate wild type enzyme, 20°C, pH 9.0 Escherichia coli
0.0107
-
NAD+ mutant Q262A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0121
-
NAD+ mutant S67A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.0122
-
NAD+ wild type enzyme, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0125
-
NAD+ mutant Y39F, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.0126
-
NAD+ mutant S67A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0136
-
L-quinate mutant Q262A, 20°C, pH 9.0 Escherichia coli
0.0142
-
NAD+ mutant Q262A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.0157
-
L-quinate mutant S67A, 20°C, pH 9.0 Escherichia coli
0.0158
-
NAD+ mutant T106A, in the presence of shihikimat, 20°C, pH 9.0 Escherichia coli
0.0158
-
NAD+ mutant Y39F, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0168
-
NAD+ mutant S22A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.018
-
NAD+ mutant S22A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.0184
-
NAD+ wild type enzyme, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.0578
-
NAD+ mutant T106A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.199
-
shikimate mutant T106A, 20°C, pH 9.0 Escherichia coli
0.513
-
shikimate mutant K71A, 20°C, pH 9.0 Escherichia coli
20.52
-
L-quinate mutant T106A, 20°C, pH 9.0 Escherichia coli
32.68
-
L-quinate mutant K71A, 20°C, pH 9.0 Escherichia coli
40.07
-
shikimate mutant D107A, 20°C, pH 9.0 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31350
-
2 * 31350, wild type enzyme, gel filtration Escherichia coli
33000
-
SDS-PAGE, mutant N92A Escherichia coli
57000
-
SDS-PAGE, wild type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6D5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydroquinate + NADPH + H+
-
Escherichia coli L-quinate + NADP+
-
r
3-dehydroshikimate + NADH + H+
-
Escherichia coli shikimate + NAD+
-
r
L-quinate + NADP+
-
Escherichia coli 3-dehydroquinate + NADPH + H+
-
r
shikimate + NAD+
-
Escherichia coli 3-dehydroshikimate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 31350, wild type enzyme, gel filtration Escherichia coli

Synonyms

Synonyms Comment Organism
quinate/shikimate 5-dehydrogenase
-
Escherichia coli
YdiB
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
NAD+ mutant K71A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.011
-
shikimate mutant K71A, 20°C, pH 9.0 Escherichia coli
0.012
-
L-quinate mutant K71A, 20°C, pH 9.0 Escherichia coli
0.012
-
NAD+ mutant K71A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.022
-
NAD+ mutant D107A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.022
-
NAD+ mutant Q262A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.023
-
NAD+ mutant S67A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.024
-
L-quinate mutant S67A, 20°C, pH 9.0 Escherichia coli
0.043
-
NAD+ mutant Q262A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.05
-
shikimate mutant Q262A, 20°C, pH 9.0 Escherichia coli
0.05
-
L-quinate mutant Q262A, 20°C, pH 9.0 Escherichia coli
0.054
-
shikimate mutant T106A, 20°C, pH 9.0 Escherichia coli
0.06
-
L-quinate mutant Y39F, 20°C, pH 9.0 Escherichia coli
0.068
-
L-quinate mutant S22A, 20°C, pH 9.0 Escherichia coli
0.082
-
NAD+ mutant S22A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.09
-
NAD+ mutant Y39F, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.091
-
shikimate wild type enzyme, 20°C, pH 9.0 Escherichia coli
0.105
-
NAD+ wild type enzyme, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.108
-
NAD+ mutant T106A, in the presence of shihikimat, 20°C, pH 9.0 Escherichia coli
0.109
-
shikimate mutant S22A, 20°C, pH 9.0 Escherichia coli
0.113
-
L-quinate wild type enzyme, 20°C, pH 9.0 Escherichia coli
0.122
-
NAD+ mutant T106A, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.132
-
shikimate mutant D107A, 20°C, pH 9.0 Escherichia coli
0.142
-
NAD+ wild type enzyme, in the presence of L-quinate, 20°C, pH 9.0 Escherichia coli
0.148
-
shikimate mutant S67A, 20°C, pH 9.0 Escherichia coli
0.153
-
NAD+ mutant S22A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.178
-
NAD+ mutant S67A, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli
0.187
-
shikimate mutant Y39F, 20°C, pH 9.0 Escherichia coli
0.233
-
L-quinate mutant T106A, 20°C, pH 9.0 Escherichia coli
0.24
-
NAD+ mutant Y39F, in the presence of shikimate, 20°C, pH 9.0 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADP+
-
Escherichia coli
NADPH
-
Escherichia coli