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Literature summary for 1.1.1.270 extracted from

  • Ferrante, T.; Barge, A.; Taramino, S.; Oliaro-Bosso, S.; Balliano, G.
    4-Methylzymosterone and other intermediates of sterol biosynthesis from yeast mutants engineered in the ERG27 gene encoding 3-ketosteroid reductase (2016), Lipids, 51, 1103-1113.
    View publication on PubMed

Application

Application Comment Organism
synthesis used of mutated 3-ketosteroid reductase (Erg27) to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially. The strategies are either incubation of cell homogenates of an Erg27-deletant strain with radioactive lanosterol, or incubation of growing cells of a strain expressing point-mutated 3-ketosteroid reductase with radioactive acetate. Chemical reduction of both radioactive and non-radioactive 3-keto sterones gives the physiological 3-beta-OH sterols, as well as the non-physiological 3-alpha-OH isomers Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
K206A strain can be used to obtain radioactive and non-radioactive intermediates of sterol biosynthesis hardly or not available commercially Saccharomyces cerevisiae
additional information labelled C-4 methylsterones can be obtained from Erg27-deletant strains by incubating a cell-free homogenate with radioactive lanosterol Saccharomyces cerevisiae
R40A strain can be used as a source of 4-methylzymosterone Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q12452
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Synonyms

Synonyms Comment Organism
Erg27
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Saccharomyces cerevisiae