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Literature summary for 1.1.1.270 extracted from

  • Jin, Y.; Penning, T.M.
    Molecular docking simulations of steroid substrates into human cytosolic hydroxysteroid dehydrogenases (AKR1C1 and AKR1C2): insights into positional and stereochemical preferences (2006), Steroids, 71, 380-391.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q04828
-
-

Reaction

Reaction Comment Organism Reaction ID
a 3beta-hydroxysteroid + NADP+ = a 3-oxosteroid + NADPH + H+ residues Leu/Val54, Tyr55, His117, Val128, Ile129, His222, and Trp227 are involved in substrate binding, Tyr55, Asp50, Lys84, and His117 form the catalytic tetrad, Tyr55 and His117 together with NADP+ form an oxyanion hole through hydrogen bond networks, molecular docking simulations, detailed overview Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0039
-
with substrate 5alpha-dihydrotestosterone, formation of the 3alpha-hydroxysteroid Homo sapiens
0.0161
-
with substrate 5alpha-dihydrotestosterone, formation of the 3beta-hydroxysteroid Homo sapiens
0.0312
-
with substrate tibolone Homo sapiens
0.0432
-
with substrate progesterone Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-dihydrotestosterone + NADPH + H+ i.e. DHT, reductive 3alpha-HSD activity of AKR1C1 yielding the 3alpha,17-diol, low activity Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NADP+
-
?
5alpha-dihydrotestosterone + NADPH + H+ i.e. DHT, reductive 3beta-HSD activity of AKR1C1 yielding the 3beta,17-diol, preferred reaction Homo sapiens 5alpha-androstan-3beta,17beta-diol + NADP+
-
?
additional information AKR1C1 is a cytosolic hydroxysteroid dehydrogenase, that reduces 3-ketosteroids and 20-ketosteroids, overview, molecular docking simulations with generation of docking targets, overview Homo sapiens ?
-
?
progesterone + NADPH i.e. pregn-4-ene-3,20-dione Homo sapiens pregn-4-ene-20alpha-ol-3-one + NADP+
-
?
[7alpha,17alpha]-17-hydroxy-7-methyl-19-norpregn-5(10)-en-20-yn-3-one + NADPH i.e. tibolone, a synthetic hormone Homo sapiens [7alpha,17alpha]-17-hydroxy-7-methyl-19-norpregn-5(10)-en-20-yn-3-ol + NADP+
-
?

Synonyms

Synonyms Comment Organism
3-ketosteroid reductase
-
Homo sapiens
3beta-hydroxysteroid dehydrogenase
-
Homo sapiens
AKR1C1
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Homo sapiens