Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ethanesulfonate | 1 mM, 5.6fold increase in ratio of turnover number to Km-value for 2-butanone as substrate | Xanthobacter autotrophicus | |
methanesulfonate | 1 mM, 5.6fold increase in ratio of turnover number to Km-value for 2-butanone as substrate | Xanthobacter autotrophicus | |
propanesulfonate | 1 mM, 1.7fold increase in ratio of turnover number to Km-value for 2-butanone as substrate | Xanthobacter autotrophicus |
Cloned (Comment) | Organism |
---|---|
- |
Xanthobacter autotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
R152A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 5192fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.3fold higher than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
R179A | no detectable activity with 2-(2-ketopropylthio)ethanesulfonate and 2-butanone | Xanthobacter autotrophicus |
R196A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 1000fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 2.7fold higher than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
R203A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 4.3fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.6fold lower than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
R209A | the ratio of turnover number to Km-value for 2-(2-ketopropylthio)ethanesulfonate is 2.7fold lower than the ratio for the wild-type enzyme. The ratio of turnover number to Km-value for 2-butanone is 1.4fold lower than the ratio for the wild-type enzyme | Xanthobacter autotrophicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.092 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
0.1 | - |
2-[(S)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.102 | - |
2-[(R)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.44 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
1.08 | - |
(R)-2-octanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
2 | 3 | 2-butanone | pH 7.5, 30°C, mutant enzyme R196A | Xanthobacter autotrophicus | |
2.64 | - |
(R)-2-heptanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
4.6 | - |
(R)-2-hexanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
20 | - |
(R)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
39 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R203A | Xanthobacter autotrophicus | |
41 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R152A | Xanthobacter autotrophicus | |
52 | - |
2-butanone | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
84 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
153 | - |
(S)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
315 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
328 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1726 | - |
2-propanol | pH 7.5, 30°C | Xanthobacter autotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xanthobacter autotrophicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2-butanol + NAD+ | the enantiomeric selectivity of the reverse reaction is 39.6% for (S)-2-butanol without additive, 90.8% with 1 mM CH3CH2SO3-Na+ as additive, 87.8% with 1 mM HSCH2CH2SO3-Na+ as additive, 52.0% with 1 mM CH3CH2COO-Na+ as additive, 46.6% with 1 mM CH3COO-Na+ as additive, 44.8% with 1 mM CH3CH2NH3+Cl- as additive, 45.4% with 1 mM CH3CH2OH as additive, 45.8% with 1 mM Na2SO4 as additive and 40.4% with 1 mM NaCl as additive | Xanthobacter autotrophicus | 2-butanone + NADH + H+ | - |
r | |
(R)-2-heptanol + NAD+ | - |
Xanthobacter autotrophicus | 2-heptanone + NADH + H+ | - |
? | |
(R)-2-hexanol + NAD+ | - |
Xanthobacter autotrophicus | 2-hexanone + NADH + H+ | - |
? | |
(R)-2-octanol + NAD+ | - |
Xanthobacter autotrophicus | 2-octanone + NADH + H+ | - |
? | |
(R)-2-pentanol + NAD+ | - |
Xanthobacter autotrophicus | 2-pentanone + NADH + H+ | - |
? | |
(S)-2-butanol + NAD+ | - |
Xanthobacter autotrophicus | 2-butanone + NADH + H+ | - |
? | |
(S)-2-pentanol + NAD+ | - |
Xanthobacter autotrophicus | 2-pentanone + NADH + H+ | - |
? | |
2-propanol + NAD+ | - |
Xanthobacter autotrophicus | acetone + NADH + H+ | - |
? | |
2-[(R)-2-hydroxypropylthio]ethanesulfonate + NAD+ | the enzyme is highly specific for the R-enantiomer | Xanthobacter autotrophicus | 2-(2-ketopropylthio)ethanesulfonate + NADH + H+ | - |
r | |
2-[(S)-2-hydroxypropylthio]ethanesulfonate + NAD+ | the enzyme is highly specific for the R-enantiomer | Xanthobacter autotrophicus | 2-(2-ketopropylthio)ethanesulfonate + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
R-HPCDH | - |
Xanthobacter autotrophicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R203A | Xanthobacter autotrophicus | |
0.027 | - |
2-butanone | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
0.028 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R152A | Xanthobacter autotrophicus | |
0.032 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
0.033 | - |
2-butanone | pH 7.5, 30°C, mutant enzyme R196A | Xanthobacter autotrophicus | |
0.044 | - |
2-[(S)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
0.98 | - |
(R)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.1 | - |
(R)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.4 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R152A | Xanthobacter autotrophicus | |
1.7 | - |
(R)-2-octanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
1.8 | - |
(R)-2-heptanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
2.2 | - |
(S)-2-butanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
2.4 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R196A | Xanthobacter autotrophicus | |
2.7 | - |
(R)-2-hexanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
2.9 | - |
2-propanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
2.9 | - |
(S)-2-pentanol | pH 7.5, 30°C | Xanthobacter autotrophicus | |
15 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R209A | Xanthobacter autotrophicus | |
24.5 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, wild-type enzyme | Xanthobacter autotrophicus | |
26.8 | - |
2-[(R)-2-hydroxypropylthio]ethanesulfonate | pH 7.5, 30°C | Xanthobacter autotrophicus | |
27.9 | - |
2-(2-ketopropylthio)ethanesulfonate | pH 7.5, 30°C, mutant enzyme R203A | Xanthobacter autotrophicus |