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Literature summary for 1.1.1.263 extracted from

  • Dambe, T.R.; Kuehn, A.M.; Brossette, T.; Giffhorn, F.; Scheidig, A.J.
    Crystal structure of NADP(H)-dependent 1,5-anhydro-D-fructose reductase from Sinorhizobium morelense at 2.2 A resolution: construction of a NADH-accepting mutant and its application in rare sugar synthesis (2006), Biochemistry, 45, 10030-10042.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ensifer adhaerens

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion Ensifer adhaerens

Protein Variants

Protein Variants Comment Organism
A13G decreased activity Ensifer adhaerens
A13G/S10G decreased activity Ensifer adhaerens
A13G/S33D increased activity Ensifer adhaerens
D176A decreased activity Ensifer adhaerens
G206I decreased activity Ensifer adhaerens
H180A decreased activity Ensifer adhaerens
K94G decreased activity Ensifer adhaerens
S10G increased activity Ensifer adhaerens
S33D decreased activity Ensifer adhaerens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADPH mutant A13G in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
0.06
-
NADPH mutant G206I in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
0.06
-
NADPH recombinant wild type enzyme in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
0.1
-
NADPH native wild type enzyme in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
0.2
-
NADPH mutant K94G in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
0.27
-
NADPH mutant S10G in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
0.38
-
NADPH mutant A13G/S10G in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
1
-
NADPH mutant A13G/S33D in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
1.1
-
NADH mutant A13G in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
1.1
-
NADH mutant A13G/S33D in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
1.2
-
NADH mutant A13G/S10G in the presence of 1,5-anhydro-D-fructose Ensifer adhaerens
3.2
-
1,5-Anhydro-D-fructose mutant A13G/S33D in the presence of NADH Ensifer adhaerens
3.5
-
1,5-Anhydro-D-fructose mutant S10G in the presence of NADPH Ensifer adhaerens
6.4
-
1,5-Anhydro-D-fructose recombinant wild type enzyme in the presence of NADPH Ensifer adhaerens
7.1
-
1,5-Anhydro-D-fructose mutant A13G/S10Gin the presence of NADPH Ensifer adhaerens
8.3
-
1,5-Anhydro-D-fructose mutant G206I in the presence of NADPH Ensifer adhaerens
8.3
-
1,5-Anhydro-D-fructose native wild type enzyme in the presence of NADPH Ensifer adhaerens
8.5
-
1,5-Anhydro-D-fructose mutant A13G in the presence of NADPH Ensifer adhaerens
8.9
-
1,5-Anhydro-D-fructose mutant H108A in the presence of NADPH Ensifer adhaerens
11.1
-
1,5-Anhydro-D-fructose mutant A13G in the presence of NADH Ensifer adhaerens
20.2
-
1,5-Anhydro-D-fructose mutant A13G/S33D in the presence of NADPH Ensifer adhaerens
22.5
-
1,5-Anhydro-D-fructose mutant K94G in the presence of NADPH Ensifer adhaerens
39
-
1,5-Anhydro-D-fructose mutant A13G/S10G in the presence of NADH Ensifer adhaerens
49
-
1,5-Anhydro-D-fructose mutant D176A in the presence of NADPH Ensifer adhaerens

Organism

Organism UniProt Comment Textmining
Ensifer adhaerens
-
-
-
Ensifer adhaerens S-30.7.5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration Ensifer adhaerens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose + NADH
-
Ensifer adhaerens 1,5-anhydro-D-mannitol + NAD+
-
?
1,5-anhydro-D-fructose + NADH
-
Ensifer adhaerens S-30.7.5 1,5-anhydro-D-mannitol + NAD+
-
?
1,5-anhydro-D-fructose + NADPH
-
Ensifer adhaerens 1,5-anhydro-D-mannitol + NADP+
-
ir
1,5-anhydro-D-fructose + NADPH
-
Ensifer adhaerens S-30.7.5 1,5-anhydro-D-mannitol + NADP+
-
ir

Synonyms

Synonyms Comment Organism
1,4-AF-reductase
-
Ensifer adhaerens
AFR
-
Ensifer adhaerens
NADP(H)-dependent 1,5-anhydro-D-fructose reductase
-
Ensifer adhaerens
NADPH-dependent anhydrofructose reductase
-
Ensifer adhaerens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
1,5-Anhydro-D-fructose mutant D176A in the presence of NADPH Ensifer adhaerens
3.7
-
1,5-Anhydro-D-fructose mutant H108A in the presence of NADPH Ensifer adhaerens
4.2
-
1,5-Anhydro-D-fructose mutant K94G in the presence of NADPH Ensifer adhaerens
5.5
-
1,5-Anhydro-D-fructose mutant A13G/S10G in the presence of NADH Ensifer adhaerens
6.3
-
1,5-Anhydro-D-fructose mutant A13G/S33D in the presence of NADPH Ensifer adhaerens
12.4
-
1,5-Anhydro-D-fructose mutant A13G in the presence of NADH Ensifer adhaerens
13.5
-
1,5-Anhydro-D-fructose mutant S33D in the presence of NADH Ensifer adhaerens
119
-
1,5-Anhydro-D-fructose mutant S10G in the presence of NADPH Ensifer adhaerens
145
-
1,5-Anhydro-D-fructose recombinant wild type enzyme in the presence of NADPH Ensifer adhaerens
156
-
1,5-Anhydro-D-fructose mutant G206I in the presence of NADPH Ensifer adhaerens
216
-
1,5-Anhydro-D-fructose native wild type enzyme in the presence of NADPH Ensifer adhaerens
369
-
1,5-Anhydro-D-fructose mutant A13G/S10Gin the presence of NADPH Ensifer adhaerens
405
-
1,5-Anhydro-D-fructose mutant A13G in the presence of NADPH Ensifer adhaerens

Cofactor

Cofactor Comment Organism Structure
NAD(P)H
-
Ensifer adhaerens