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Literature summary for 1.1.1.263 extracted from

  • Kuehn, A.; Yu, S.; Giffhorn, F.
    Catabolism of 1,5-anhydro-D-fructose in Sinorhizobium morelense S-30.7.5: discovery, characterization, and overexpression of a new 1,5-anhydro-D-fructose reductase and its application in sugar analysis and rare sugar synthesis (2006), Appl. Environ. Microbiol., 72, 1248-1257.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information activity of AFR is not influenced by 1 mM EDTA Ensifer adhaerens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ensifer adhaerens

General Stability

General Stability Organism
the highest activity of AFR is measured at 30°C in 100 mM Bistris-HCl at pH 6.5, with 50% remaining activity at pH 5.2 (in citrate buffer) and pH 8.8 (in Tris-HCl buffer) Ensifer adhaerens

Inhibitors

Inhibitors Comment Organism Structure
additional information activity of AFR was not influenced by 1 mM EDTA or by any of the given metal salts NaCl, KCl, MgCl2, CaCl2, MnCl2, FeCl3, and ZnCl2, 1 mM each Ensifer adhaerens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
NADPH
-
Ensifer adhaerens
8.4
-
1,5-Anhydro-D-fructose
-
Ensifer adhaerens
11
-
D-glucosone
-
Ensifer adhaerens

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity of AFR was not influenced by NaCl, KCl, MgCl2, CaCl2, MnCl2, FeCl3, and ZnCl2, 1 mM each Ensifer adhaerens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35100
-
MALDI-TOF-MS Ensifer adhaerens
38200
-
gel filtration Ensifer adhaerens
40000
-
SDS-PAGE Ensifer adhaerens

Organism

Organism UniProt Comment Textmining
Ensifer adhaerens
-
-
-
Ensifer adhaerens S-30.7.5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose HP column chromatography, Superdex 200 HR10 30 column chromatography and Red Sepharose CL-6B chromatography Ensifer adhaerens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
AFR of S. morelense S-30.7.5 was inactive toward pyridine-3-aldehyde, 2,3-butanedione, glucuronic acid, acetaldehyde, and formaldehyde Ensifer adhaerens
0.01
-
1,5-anhydro-D-glucitol as substrate Ensifer adhaerens
0.06
-
1,5-anhydro-D-mannitol as substrate Ensifer adhaerens
6.5
-
in crude cell extract Ensifer adhaerens
21
-
ascopyrone as substrate Ensifer adhaerens
21
-
D-galactosone as substrate Ensifer adhaerens
53
-
D-allosone as substrate Ensifer adhaerens
88
-
D-xylosone as substrate Ensifer adhaerens
118
-
6-deoxy-D-glucosone as substrate Ensifer adhaerens
118
-
D-glucosone as substrate Ensifer adhaerens
191
-
3-keto-1,5-anhydro-D-fructose as substrate Ensifer adhaerens
488.9
-
after 75 fold purification Ensifer adhaerens
530
-
1,5-anhydro-D-fructose as substrate Ensifer adhaerens

Storage Stability

Storage Stability Organism
-20°C, 50 days, 50% loss of activity Ensifer adhaerens
0°C, 50 days, 50% loss of activity Ensifer adhaerens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,5-anhydro-D-fructose + NADP+
-
Ensifer adhaerens 1,5-anhydro-D-mannitol + NADPH
-
r
1,5-anhydro-D-fructose + NADP+
-
Ensifer adhaerens S-30.7.5 1,5-anhydro-D-mannitol + NADPH
-
r
1,5-anhydro-D-glucitol + NADPH
-
Ensifer adhaerens ? + NADP+
-
?
1,5-anhydro-D-glucitol + NADPH
-
Ensifer adhaerens S-30.7.5 ? + NADP+
-
?
1,5-anhydro-D-mannitol + NADPH
-
Ensifer adhaerens ? + NADP+
-
?
1,5-anhydro-D-mannitol + NADPH
-
Ensifer adhaerens S-30.7.5 ? + NADP+
-
?
3-keto-1,5-anhydro-D-fructose + NADP+
-
Ensifer adhaerens 1,5-anhydro-D-mannitol + NADPH
-
?
3-keto-1,5-anhydro-D-fructose + NADP+
-
Ensifer adhaerens S-30.7.5 1,5-anhydro-D-mannitol + NADPH
-
?
6-deoxy-D-glucosone + NADP+
-
Ensifer adhaerens 6-deoxy-D-mannose + NADPH
-
?
6-deoxy-D-glucosone + NADP+
-
Ensifer adhaerens S-30.7.5 6-deoxy-D-mannose + NADPH
-
?
ascopyrone P + NADP+
-
Ensifer adhaerens ? + NADPH
-
?
D-allosone + NADP+
-
Ensifer adhaerens D-altrose + NADPH
-
?
D-galactosone + NADP+
-
Ensifer adhaerens D-talose + NADPH
-
?
D-glucosone + NADP+
-
Ensifer adhaerens D-mannose + NADPH
-
?
D-xylosone + NADP+
-
Ensifer adhaerens D-lyxose + NADPH
-
?

Subunits

Subunits Comment Organism
monomer 1 * 35100, MALDI-TOF-MS Ensifer adhaerens

Synonyms

Synonyms Comment Organism
AFR
-
Ensifer adhaerens
NADPH-dependent monomeric reductase
-
Ensifer adhaerens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
63.2
-
D-glucosone
-
Ensifer adhaerens
286.7
-
1,5-Anhydro-D-fructose
-
Ensifer adhaerens
1300
-
1,5-Anhydro-D-fructose
-
Ensifer adhaerens

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 8.8 at 30°C the highest activity of AFR is measured in 100 mM Bistris-HCl at pH 6.5, with 50% remaining activity at pH 5.2 (in citrate buffer) and pH 8.8 (in Tris-HCl buffer) Ensifer adhaerens

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Ensifer adhaerens

pI Value

Organism Comment pI Value Maximum pI Value
Ensifer adhaerens
-
-
4.3