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Literature summary for 1.1.1.261 extracted from

  • Carbone, V.; Schofield, L.R.; Zhang, Y.; Sang, C.; Dey, D.; Hannus, I.M.; Martin, W.F.; Sutherland-Smith, A.J.; Ronimus, R.S.
    Structure and evolution of the archaeal lipid synthesis enzyme sn-glycerol-1-phosphate dehydrogenase (2015), J. Biol. Chem., 290, 21690-21704.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
KCl an optimum concentration of about 150 mM KCl produces a more than 400% increase in activity compared with an absence of KCl Methanocaldococcus jannaschii
KCl the optimum concentration of about 150 mM KCl produces more than 400% increase in activity Methanocaldococcus jannaschii

Cloned(Commentary)

Cloned (Comment) Organism
-
Methanocaldococcus jannaschii
expressed in Escherichia coli Rosetta 2 (DE3) cells Methanocaldococcus jannaschii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 30% (w/v) poly(ethylene glycol) methyl ether 550/polyethylene glycol 20000 and 0.1 M HEPES, pH 7.6 Methanocaldococcus jannaschii
structure with bound substrate dihydroxyacetone phosphate, product glycerol 1-phosphate, NADPH, and Zn2+ cofactor. The sole role of His226, His247, and Asp148 is to coordinate the active site Zn2+. A pro-R hydride transfer/relay system is in operation during catalysis, mediated by water and completed by the polarization of the carbonyl of dihydroxyacetone phosphate by Zn2+. Residue Asn104 may influence affinity for the coenzyme and the rate of reaction via its interaction with NADPH Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0432
-
NADPH pH 7.0, 50°C Methanocaldococcus jannaschii
0.0432
-
NADPH at pH 7.8 and 65°C Methanocaldococcus jannaschii
0.141
-
NADH pH 7.0, 50°C Methanocaldococcus jannaschii
0.141
-
NADH at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.11
-
dihydroxyacetone phosphate cosubstrate NADH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.11
-
dihydroxyacetone phosphate with NADH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
2.05
-
dihydroxyacetone phosphate cosubstrate NADPH, pH 7.0, 50°C Methanocaldococcus jannaschii
2.05
-
dihydroxyacetone phosphate with NADPH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Cd2+ about 20% of the activity with Zn2+ Methanocaldococcus jannaschii
Co2+ about 150% of the activity with Zn2+ Methanocaldococcus jannaschii
Fe2+ dependent on Methanocaldococcus jannaschii
Mg2+ about 20% of the activity with Zn2+ Methanocaldococcus jannaschii
Mn2+ about 20% of the activity with Zn2+ Methanocaldococcus jannaschii
Zn2+ a single Zn2+ ion is bound in each glycerol-1-phosphate dehydrogenase molecule forming bonds to His226, His247, and Asp148 Methanocaldococcus jannaschii
Zn2+ required for activity, 0.1 mM is the optimum concentration Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
1 * 43000, SDS-PAGE Methanocaldococcus jannaschii
44000
-
gel filtration Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58122
-
-
Methanocaldococcus jannaschii DSM 2661 Q58122
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroxyacetone phosphate + NADH + H+
-
Methanocaldococcus jannaschii sn-glycerol 1-phosphate + NAD+
-
r
dihydroxyacetone phosphate + NADH + H+
-
Methanocaldococcus jannaschii DSM 2661 sn-glycerol 1-phosphate + NAD+
-
r
dihydroxyacetone phosphate + NADH + H+
-
Methanocaldococcus jannaschii sn-glycerol-1-phosphate + NAD+
-
?
dihydroxyacetone phosphate + NADH + H+
-
Methanocaldococcus jannaschii DSM 2661 sn-glycerol-1-phosphate + NAD+
-
?
dihydroxyacetone phosphate + NADPH + H+
-
Methanocaldococcus jannaschii sn-glycerol 1-phosphate + NADP+
-
r
dihydroxyacetone phosphate + NADPH + H+
-
Methanocaldococcus jannaschii DSM 2661 sn-glycerol 1-phosphate + NADP+
-
r
dihydroxyacetone phosphate + NADPH + H+
-
Methanocaldococcus jannaschii sn-glycerol-1-phosphate + NADP+
-
?
dihydroxyacetone phosphate + NADPH + H+
-
Methanocaldococcus jannaschii DSM 2661 sn-glycerol-1-phosphate + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 43000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
EgsA
-
Methanocaldococcus jannaschii
G1PDH
-
Methanocaldococcus jannaschii
NAD(P)H-dependent G1P dehydrogenase
-
Methanocaldococcus jannaschii
sn-glycerol-1-phosphate dehydrogenase
-
Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.44
-
dihydroxyacetone phosphate cosubstrate NADH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.44
-
dihydroxyacetone phosphate with NADH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
2.78
-
dihydroxyacetone phosphate cosubstrate NADPH, pH 7.0, 50°C Methanocaldococcus jannaschii
2.78
-
dihydroxyacetone phosphate with NADPH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
3.23
-
NADPH pH 7.0, 50°C Methanocaldococcus jannaschii
3.23
-
NADPH at pH 7.8 and 65°C Methanocaldococcus jannaschii
3.33
-
NADH pH 7.0, 50°C Methanocaldococcus jannaschii
3.33
-
NADH at pH 7.8 and 65°C Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7.5
-
Methanocaldococcus jannaschii
6.5 7.5 broad Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methanocaldococcus jannaschii
NADPH
-
Methanocaldococcus jannaschii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.29
-
dihydroxyacetone phosphate cosubstrate NADH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.295
-
dihydroxyacetone phosphate with NADH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
1.36
-
dihydroxyacetone phosphate cosubstrate NADPH, pH 7.0, 50°C Methanocaldococcus jannaschii
1.36
-
dihydroxyacetone phosphate with NADPH as cosubstrate, at pH 7.8 and 65°C Methanocaldococcus jannaschii
23.67
-
NADH pH 7.0, 50°C Methanocaldococcus jannaschii
23.67
-
NADH at pH 7.8 and 65°C Methanocaldococcus jannaschii
74.67
-
NADPH pH 7.0, 50°C Methanocaldococcus jannaschii
74.67
-
NADPH at pH 7.8 and 65°C Methanocaldococcus jannaschii