Activating Compound | Comment | Organism | Structure |
---|---|---|---|
KCl | an optimum concentration of about 150 mM KCl produces a more than 400% increase in activity compared with an absence of KCl | Methanocaldococcus jannaschii | |
KCl | the optimum concentration of about 150 mM KCl produces more than 400% increase in activity | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
expressed in Escherichia coli Rosetta 2 (DE3) cells | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) poly(ethylene glycol) methyl ether 550/polyethylene glycol 20000 and 0.1 M HEPES, pH 7.6 | Methanocaldococcus jannaschii |
structure with bound substrate dihydroxyacetone phosphate, product glycerol 1-phosphate, NADPH, and Zn2+ cofactor. The sole role of His226, His247, and Asp148 is to coordinate the active site Zn2+. A pro-R hydride transfer/relay system is in operation during catalysis, mediated by water and completed by the polarization of the carbonyl of dihydroxyacetone phosphate by Zn2+. Residue Asn104 may influence affinity for the coenzyme and the rate of reaction via its interaction with NADPH | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0432 | - |
NADPH | pH 7.0, 50°C | Methanocaldococcus jannaschii | |
0.0432 | - |
NADPH | at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
0.141 | - |
NADH | pH 7.0, 50°C | Methanocaldococcus jannaschii | |
0.141 | - |
NADH | at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
1.11 | - |
dihydroxyacetone phosphate | cosubstrate NADH, pH 7.0, 50°C | Methanocaldococcus jannaschii | |
1.11 | - |
dihydroxyacetone phosphate | with NADH as cosubstrate, at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
2.05 | - |
dihydroxyacetone phosphate | cosubstrate NADPH, pH 7.0, 50°C | Methanocaldococcus jannaschii | |
2.05 | - |
dihydroxyacetone phosphate | with NADPH as cosubstrate, at pH 7.8 and 65°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | about 20% of the activity with Zn2+ | Methanocaldococcus jannaschii | |
Co2+ | about 150% of the activity with Zn2+ | Methanocaldococcus jannaschii | |
Fe2+ | dependent on | Methanocaldococcus jannaschii | |
Mg2+ | about 20% of the activity with Zn2+ | Methanocaldococcus jannaschii | |
Mn2+ | about 20% of the activity with Zn2+ | Methanocaldococcus jannaschii | |
Zn2+ | a single Zn2+ ion is bound in each glycerol-1-phosphate dehydrogenase molecule forming bonds to His226, His247, and Asp148 | Methanocaldococcus jannaschii | |
Zn2+ | required for activity, 0.1 mM is the optimum concentration | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
1 * 43000, SDS-PAGE | Methanocaldococcus jannaschii |
44000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58122 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58122 | - |
- |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydroxyacetone phosphate + NADH + H+ | - |
Methanocaldococcus jannaschii | sn-glycerol 1-phosphate + NAD+ | - |
r | |
dihydroxyacetone phosphate + NADH + H+ | - |
Methanocaldococcus jannaschii DSM 2661 | sn-glycerol 1-phosphate + NAD+ | - |
r | |
dihydroxyacetone phosphate + NADH + H+ | - |
Methanocaldococcus jannaschii | sn-glycerol-1-phosphate + NAD+ | - |
? | |
dihydroxyacetone phosphate + NADH + H+ | - |
Methanocaldococcus jannaschii DSM 2661 | sn-glycerol-1-phosphate + NAD+ | - |
? | |
dihydroxyacetone phosphate + NADPH + H+ | - |
Methanocaldococcus jannaschii | sn-glycerol 1-phosphate + NADP+ | - |
r | |
dihydroxyacetone phosphate + NADPH + H+ | - |
Methanocaldococcus jannaschii DSM 2661 | sn-glycerol 1-phosphate + NADP+ | - |
r | |
dihydroxyacetone phosphate + NADPH + H+ | - |
Methanocaldococcus jannaschii | sn-glycerol-1-phosphate + NADP+ | - |
? | |
dihydroxyacetone phosphate + NADPH + H+ | - |
Methanocaldococcus jannaschii DSM 2661 | sn-glycerol-1-phosphate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
EgsA | - |
Methanocaldococcus jannaschii |
G1PDH | - |
Methanocaldococcus jannaschii |
NAD(P)H-dependent G1P dehydrogenase | - |
Methanocaldococcus jannaschii |
sn-glycerol-1-phosphate dehydrogenase | - |
Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.44 | - |
dihydroxyacetone phosphate | cosubstrate NADH, pH 7.0, 50°C | Methanocaldococcus jannaschii | |
1.44 | - |
dihydroxyacetone phosphate | with NADH as cosubstrate, at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
2.78 | - |
dihydroxyacetone phosphate | cosubstrate NADPH, pH 7.0, 50°C | Methanocaldococcus jannaschii | |
2.78 | - |
dihydroxyacetone phosphate | with NADPH as cosubstrate, at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
3.23 | - |
NADPH | pH 7.0, 50°C | Methanocaldococcus jannaschii | |
3.23 | - |
NADPH | at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
3.33 | - |
NADH | pH 7.0, 50°C | Methanocaldococcus jannaschii | |
3.33 | - |
NADH | at pH 7.8 and 65°C | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Methanocaldococcus jannaschii |
6.5 | 7.5 | broad | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methanocaldococcus jannaschii | |
NADPH | - |
Methanocaldococcus jannaschii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.29 | - |
dihydroxyacetone phosphate | cosubstrate NADH, pH 7.0, 50°C | Methanocaldococcus jannaschii | |
1.295 | - |
dihydroxyacetone phosphate | with NADH as cosubstrate, at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
1.36 | - |
dihydroxyacetone phosphate | cosubstrate NADPH, pH 7.0, 50°C | Methanocaldococcus jannaschii | |
1.36 | - |
dihydroxyacetone phosphate | with NADPH as cosubstrate, at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
23.67 | - |
NADH | pH 7.0, 50°C | Methanocaldococcus jannaschii | |
23.67 | - |
NADH | at pH 7.8 and 65°C | Methanocaldococcus jannaschii | |
74.67 | - |
NADPH | pH 7.0, 50°C | Methanocaldococcus jannaschii | |
74.67 | - |
NADPH | at pH 7.8 and 65°C | Methanocaldococcus jannaschii |