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Literature summary for 1.1.1.255 extracted from

  • Stoop, J.M.H.; Williamson, J.D.; Conkling, M.A.; MacKay, J.J.; Pharr, D.M.
    Characterization of NAD-dependent mannitol dehydrogenase from celery as affected by ions, chelators, reducing agents and metabolites (1998), Plant Sci., 131, 43-51.
No PubMed abstract available
Search for more literature for 1.1.1.255

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline dose dependent decrease in enzyme activity Apium graveolens
1,4-dithioerythritol 1.09 mM, 50% inhibition, partially reversed by diamide Apium graveolens
1,4-dithiothreitol 0.57 mM, 50% inhibition, reversed by diamide Apium graveolens
2-mercaptoethanol 30 mM, 50% inhibition, partially reversed by diamide Apium graveolens
ADP Ki: 0.24 mM at pH 7.0 Apium graveolens
AMP Ki: 0.50-0.80 mM at pH 9.5, Ki = 0.64 mM at pH 7.0 Apium graveolens
ATP Ki: 1.10 mM at pH 7.0 Apium graveolens
additional information EDTA and EGTA did not affect enzyme activity Apium graveolens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00127
-
 NADH
-
 Apium graveolens
0.14
-
 NAD+ at pH 9.5 Apium graveolens
0.23
-
 NAD+ at pH 7.0 Apium graveolens
64
-
 D-mannitol at pH 9.5 Apium graveolens
400
-
 D-mannitol at pH 7.0 Apium graveolens
745
-
 D-mannose
-
 Apium graveolens

Organism

Organism UniProt Comment Textmining
Apium graveolens
-
 L. var. dulce Mill. Pers.
-
Apium graveolens
-
 celery
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.9
-
 at pH 9.5 Apium graveolens
2.2
-
 at pH 7.0 Apium graveolens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-mannitol + NAD+
-
 Apium graveolens D-mannose + NADH + H+
-
 r
additional information no cinnamyl alcohol dehydrogenase activity Apium graveolens ?
-
 ?

Synonyms

Synonyms Comment Organism
MTD
-
 Apium graveolens

pH Range

pH Minimum pH Maximum Comment Organism
6.5
-
 D-mannose reduction Apium graveolens
9.5
-
 D-mannitol oxidation Apium graveolens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Apium graveolens