Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-endo-hydroxycineole + NAD(P)+ | Rhodococcus sp. | involved in 1,8-cineole metabolism | 6-oxocineole + NAD(P)H | - |
r | |
6-endo-hydroxycineole + NAD(P)+ | Rhodococcus sp. C1 | involved in 1,8-cineole metabolism | 6-oxocineole + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
- |
- |
Rhodococcus sp. C1 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.62 | - |
6-endo-hydroxycineole | Rhodococcus sp. |
1.08 | - |
6-oxocineole | Rhodococcus sp. |
Storage Stability | Organism |
---|---|
-20°C, after dialysis against 1 mM KH2PO4/Na2HPO4 buffer, pH 7.1 | Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-endo-hydroxycineole + NAD(P)+ | 10% of activity with NADPH over NADH in reverse reaction | Rhodococcus sp. | 6-oxocineole + NAD(P)H | - |
r | |
6-endo-hydroxycineole + NAD(P)+ | involved in 1,8-cineole metabolism | Rhodococcus sp. | 6-oxocineole + NAD(P)H | - |
r | |
6-endo-hydroxycineole + NAD(P)+ | 10% of activity with NADPH over NADH in reverse reaction | Rhodococcus sp. C1 | 6-oxocineole + NAD(P)H | - |
r | |
6-endo-hydroxycineole + NAD(P)+ | involved in 1,8-cineole metabolism | Rhodococcus sp. C1 | 6-oxocineole + NAD(P)H | - |
r |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
NADH oxidation | Rhodococcus sp. |
10.5 | - |
NAD+ reduction | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | NADH in reverse reaction | Rhodococcus sp. | |
NADP+ | NADPH in reverse reaction, 10% of activity over NADH | Rhodococcus sp. |