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Literature summary for 1.1.1.219 extracted from

  • Chemler, J.A.; Fowler, Z.L.; McHugh, K.P.; Koffas, M.A.
    Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering (2009), Metab. Eng., 12, 96-104.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli strains transformed with plasmid pTrcHis2-DFR to only express DFR. Escherichia coli strains harboring plasmid pET-DFR-LAR expressing DFR and leucoanthocyanidin reductase Anthurium andraeanum

Organism

Organism UniProt Comment Textmining
Anthurium andraeanum Q84L22
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydroquercetin + NADPH + H+ maximum detected levels of extracellular leucocyanidin produced from Escherichia coli strains BL21StarTM (DE3), BLDELTApgi, BLDELTApgiDELTAppc, BLDELTApgiDELTApldADELTAppc and BLDELTApgiDELTApldBDELTAppc expressing DFR Anthurium andraeanum leucocyanidin + NADP+
-
r

Synonyms

Synonyms Comment Organism
DFR
-
Anthurium andraeanum
dihydroflavonol 4-reductase
-
Anthurium andraeanum

Cofactor

Cofactor Comment Organism Structure
NADPH conversion of dihydroquercetin by DFR is likely dependent on the [NADPH]/[NADP+] ratio and less so on the absolute value of NADPH within the cell Anthurium andraeanum

General Information

General Information Comment Organism
physiological function synthesis of(+)-catechin by leucoanthocyanidin reductase may be tied to regulation of DFR Anthurium andraeanum