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Literature summary for 1.1.1.219 extracted from

  • Leonard, E.; Yan, Y.; Chemler, J.; Matern, U.; Martens, S.; Koffas, M.
    Characterization of dihydroflavonol 4-reductases for recombinant plant pigment biosynthesis applications (2008), Biocatal. Biotransform., 26, 243-251.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Rosa hybrid cultivar
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Arabidopsis thaliana
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Anthurium andraeanum
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Lilium sp.
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Fragaria x ananassa
into pTrcHis2-TOPO and heterologously expressed in Escherichia coli TOP10F' strain. DFR cDNA cloned into pRSF-FHT and inserted into Escherichia coli BL21Star to create E-color strain Ipomoea nil

Organism

Organism UniProt Comment Textmining
Anthurium andraeanum Q84L22
-
-
Arabidopsis thaliana P51102
-
-
Fragaria x ananassa O22617
-
-
Ipomoea nil O24607
-
-
Lilium sp. Q6UAQ7 hybrid, cv. Star Gazer
-
Rosa hybrid cultivar Q41158 cv. minirose
-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Rosa hybrid cultivar
-
flower
-
Lilium sp.
-
fruit
-
Fragaria x ananassa
-
additional information spathe Anthurium andraeanum
-
petal
-
Rosa hybrid cultivar
-
petal
-
Lilium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00002
-
with dihydrokaempferol as substrate Arabidopsis thaliana
0.0003
-
with dihydrokaempferol as substrate Ipomoea nil
0.0003
-
with naringenin as substrate Ipomoea nil
0.0004
-
with dihydrokaempferol as substrate Rosa hybrid cultivar
0.0004
-
with naringenin as substrate Rosa hybrid cultivar
0.0005
-
with naringenin as substrate Fragaria x ananassa
0.0006
-
with eriodictyol as substrate Arabidopsis thaliana
0.0006
-
with eriodictyol as substrate Lilium sp.
0.0006
-
with eriodictyol as substrate Fragaria x ananassa
0.0006
-
with eriodictyol as substrate Ipomoea nil
0.0007
-
with eriodictyol as substrate Rosa hybrid cultivar
0.0007
-
with naringenin as substrate Anthurium andraeanum
0.0008
-
with eriodictyol as substrate Anthurium andraeanum
0.001
-
with dihydromyricetin as substrate Arabidopsis thaliana
0.001
-
with dihydroquercitin as substrate Rosa hybrid cultivar
0.0012
-
with dihydromyricetin as substrate Lilium sp.
0.0012
-
with dihydroquercitin as substrate Fragaria x ananassa
0.0013
-
with dihydromyricetin as substrate Rosa hybrid cultivar
0.0013
-
with dihydroquercitin as substrate Arabidopsis thaliana
0.0013
-
with dihydroquercitin as substrate Lilium sp.
0.0013
-
with dihydroquercitin as substrate Ipomoea nil
0.0014
-
with dihydromyricetin as substrate Anthurium andraeanum
0.0014
-
with dihydromyricetin as substrate Fragaria x ananassa
0.0014
-
with dihydroquercitin as substrate Anthurium andraeanum
0.0015
-
with dihydromyricetin as substrate Ipomoea nil
0.014
-
with dihydrokaempferol as substrate Fragaria x ananassa
0.021
-
with dihydrokaempferol as substrate Anthurium andraeanum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-hydroxyflavanone + NADPH + H+
-
Anthurium andraeanum 2,4-cis-7-hydroxyflavan-4-ol + 2,4-trans-7-hydroxyflavan-4-ol + NADP+
-
?
dihydrokaempferol + NADPH + H+
-
Rosa hybrid cultivar leucopelargonidin + NADP+
-
?
dihydrokaempferol + NADPH + H+
-
Arabidopsis thaliana leucopelargonidin + NADP+
-
?
dihydrokaempferol + NADPH + H+
-
Fragaria x ananassa leucopelargonidin + NADP+
-
?
dihydrokaempferol + NADPH + H+
-
Ipomoea nil leucopelargonidin + NADP+
-
?
dihydrokaempferol + NADPH + H+
-
Anthurium andraeanum leucopelargonidin + NADP+ highest pelargonidin concentration derived from the E-color culture harboring the Anthurium andraeanum DFR ?
dihydromyricetin + NADPH + H+
-
Rosa hybrid cultivar leucodelphinidin + NADP+
-
?
dihydromyricetin + NADPH + H+
-
Arabidopsis thaliana leucodelphinidin + NADP+
-
?
dihydromyricetin + NADPH + H+
-
Anthurium andraeanum leucodelphinidin + NADP+
-
?
dihydromyricetin + NADPH + H+
-
Lilium sp. leucodelphinidin + NADP+
-
?
dihydromyricetin + NADPH + H+
-
Fragaria x ananassa leucodelphinidin + NADP+
-
?
dihydromyricetin + NADPH + H+
-
Ipomoea nil leucodelphinidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Rosa hybrid cultivar leucocyanidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Arabidopsis thaliana leucocyanidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Anthurium andraeanum leucocyanidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Lilium sp. leucocyanidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Fragaria x ananassa leucocyanidin + NADP+
-
?
dihydroquercetin + NADPH + H+
-
Ipomoea nil leucocyanidin + NADP+
-
?
eriodictyol + NADPH + H+
-
Rosa hybrid cultivar luteoforol + NADP+
-
?
eriodictyol + NADPH + H+
-
Anthurium andraeanum luteoforol + NADP+
-
?
eriodictyol + NADPH + H+
-
Lilium sp. luteoforol + NADP+
-
?
eriodictyol + NADPH + H+
-
Fragaria x ananassa luteoforol + NADP+
-
?
eriodictyol + NADPH + H+
-
Ipomoea nil luteoforol + NADP+
-
?
additional information can not reduce hesperetin or 5,7-dimethoxyflavanone Anthurium andraeanum ?
-
?
additional information does not catalyze dihydrokaempferol and naringenin Lilium sp. ?
-
?
additional information does not catalyze naringenin Arabidopsis thaliana ?
-
?
naringenin + NADPH + H+
-
Rosa hybrid cultivar apiferol + NADP+
-
?
naringenin + NADPH + H+
-
Anthurium andraeanum apiferol + NADP+
-
?
naringenin + NADPH + H+
-
Fragaria x ananassa apiferol + NADP+
-
?
naringenin + NADPH + H+
-
Ipomoea nil apiferol + NADP+
-
?

Synonyms

Synonyms Comment Organism
DFR
-
Rosa hybrid cultivar
DFR
-
Arabidopsis thaliana
DFR
-
Anthurium andraeanum
DFR
-
Lilium sp.
DFR
-
Fragaria x ananassa
DFR
-
Ipomoea nil
dihydroflavonol 4-reductase
-
Rosa hybrid cultivar
dihydroflavonol 4-reductase
-
Arabidopsis thaliana
dihydroflavonol 4-reductase
-
Anthurium andraeanum
dihydroflavonol 4-reductase
-
Lilium sp.
dihydroflavonol 4-reductase
-
Fragaria x ananassa
dihydroflavonol 4-reductase
-
Ipomoea nil

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 30 maximum DFR activities achieved towards dihydroflavonols and flavanones Anthurium andraeanum

pH Range

pH Minimum pH Maximum Comment Organism
6 7 maximum DFR activities achieved towards dihydroflavonols and flavanones Anthurium andraeanum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Rosa hybrid cultivar
NADPH
-
Arabidopsis thaliana
NADPH
-
Anthurium andraeanum
NADPH
-
Lilium sp.
NADPH
-
Fragaria x ananassa
NADPH
-
Ipomoea nil

General Information

General Information Comment Organism
physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Rosa hybrid cultivar
physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Arabidopsis thaliana
physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Anthurium andraeanum
physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Lilium sp.
physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Fragaria x ananassa
physiological function DFR plays a key role in determining intensity and pigment coloration because its specificity and activities dictate the type and amount of the colorless leucoanthocyanidins Ipomoea nil