Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Vitis vinifera |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with NADPH and dihydroquercetin, X-ray diffraction structure determination and analysis at 1.8 A resolution | Vitis vinifera |
Protein Variants | Comment | Organism |
---|---|---|
N133D | site-directed mutagenesis, | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + NADPH | Vitis vinifera | - |
cis-3,4-leucopelargonidin + NADP+ | - |
r | |
dihydromyricetin + NADPH | Vitis vinifera | - |
leucodelphinidin + NADP+ | - |
r | |
dihydroquercetin + NADPH | Vitis vinifera | - |
leucocyanidin + NADP+ | - |
r | |
additional information | Vitis vinifera | the key enzyme in flavonoid biosynthesis catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinanet enzyme from Escherichia coli | Vitis vinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrokaempferol + NADPH | - |
Vitis vinifera | cis-3,4-leucopelargonidin + NADP+ | - |
r | |
dihydromyricetin + NADPH | - |
Vitis vinifera | leucodelphinidin + NADP+ | - |
r | |
dihydroquercetin + NADPH | - |
Vitis vinifera | leucocyanidin + NADP+ | - |
r | |
additional information | the key enzyme in flavonoid biosynthesis catalyzes a late step in the biosynthesis of anthocyanins and condensed tannins, two flavonoid classes of importance to plant survival and human nutrition, overview | Vitis vinifera | ? | - |
? | |
additional information | the specific residue at position 133, Asn or Asp, is involved in controling of the substrate recognition and recognition of the B-ring hydroxylation pattern of dihydroflavonols, structure-function relationship, overview | Vitis vinifera | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | 3D structure of the ternary complex, overview | Vitis vinifera |
Synonyms | Comment | Organism |
---|---|---|
DFR | - |
Vitis vinifera |
dihydroflavonol 4-reductase | - |
Vitis vinifera |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | binding site structure, overview | Vitis vinifera | |
NADPH | - |
Vitis vinifera |