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Literature summary for 1.1.1.219 extracted from

  • Ishikura, N.; Murakami, H.; Fujii, Y.
    Conversion of (+)-dihydroquercetin to 3,4-cis-leucocyanidin by a reductase extracted from cell suspension cultures of Cryptomeria japonica (1988), Plant Cell Physiol., 29, 795-799.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ strong inhibitor Cryptomeria japonica
iodoacetate to some extent Cryptomeria japonica
additional information no inhibition with PCMB, EDTA, Mg2+, Co2+, Fe2+, Ca2+, Mn2+, Zn2+ Cryptomeria japonica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.048
-
(+)-dihydroquercetin
-
Cryptomeria japonica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
133000
-
gel filtration with Sephadex G-150 Cryptomeria japonica

Organism

Organism UniProt Comment Textmining
Cryptomeria japonica
-
cv.'kumotoshi', japanese cedar
-

Purification (Commentary)

Purification (Comment) Organism
-
Cryptomeria japonica

Source Tissue

Source Tissue Comment Organism Textmining
callus
-
Cryptomeria japonica
-
cell suspension culture from young shoot apex Cryptomeria japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
crude extract 12.1 microgram per h and mg protein Cryptomeria japonica

Storage Stability

Storage Stability Organism
-20°C, 20% loss of activity within 2 days, 2-mercaptoethanol protects Cryptomeria japonica
-20°C, 57% loss of activity after 4 months, 2-mercaptoethanol protects Cryptomeria japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-dihydrokaempferol + NADPH 30% as active as dihydroquercetin Cryptomeria japonica cis-3,4-leucopelargonidin + NADP+
-
?
(+)-dihydroquercetin + NADPH best substrate Cryptomeria japonica cis-3,4-leucocyanidin + NADP+
-
?
additional information no substrates: (+)-dihydromorin, i.e. 3,5,7,2',4'-pentahydroxyflavanone, and pinobanksin, i.e. 3,5,7-trihydroxyflavanone Cryptomeria japonica ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Cryptomeria japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
broad optimum with Tris buffer, sharp optimum with citric acid-sodium phosphate buffer Cryptomeria japonica

Cofactor

Cofactor Comment Organism Structure
NADH cannot replace NADPH Cryptomeria japonica
NADPH
-
Cryptomeria japonica