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Literature summary for 1.1.1.218 extracted from
- Purification, characterization and partial primary structure of morphine 6-dehydrogenase from rabbit liver cytosol (1997), Arch. Biochem. Biophys., 341, 81-88.
General Stability
| General Stability | Organism |
|---|---|
| enzyme stable without 2-mercaptoethanol | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5beta-dihydrotestosterone | - |
Oryctolagus cuniculus |  |
| additional information | 5beta-dihydrotestosterone, lithocholic acid, androsterone and estradiol inhibit competitively toward morphine binding; little inhibitory effect: indomethacin, prostaglandins E2, D2; no or weak inhibition by pyrazole, barbital | Oryctolagus cuniculus | |
| NEM | - |
Oryctolagus cuniculus | |
| p-chloromercuribenzoic acid | - |
Oryctolagus cuniculus | |
| Phenylarsine oxide | - |
Oryctolagus cuniculus | |
| quercetin | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
codeine | with NAD+ | Oryctolagus cuniculus | |
| 0.12 | - |
1-Indanol | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 0.39 | - |
Dihydrocodeine | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 0.54 | - |
cyclohexene-2-ol | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 0.64 | - |
normorphine | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 0.72 | - |
morphine | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 0.92 | - |
3-hydroxyhexobarbital | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 1.48 | - |
ethylmorphine | cosubstrate NAD+ | Oryctolagus cuniculus | |
| 1.75 | - |
benzenedihydrodiol | cosubstrate NAD+ | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Oryctolagus cuniculus | 5829 | - |
| microsome | microsomal fraction | Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 31000 | - |
native enzyme, gel filtration | Oryctolagus cuniculus |
| 36000 | - |
denatured enzyme, SDS-PAGE | Oryctolagus cuniculus |
| 38000 | - |
1 * 38000, SDS-PAGE | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
Japanese white rabbit | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| gel filtration, various chromatographic techniques | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.43 | - |
morphine, NAD+ | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, 20% glycerol, v/v, stable at least four months | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-indanol + NADP+ | - |
Oryctolagus cuniculus | 1-indanone + NADPH | - |
? | |
| 3-hydroxyhexobarbital + NADP+ | does not serve as a substrate for guinea pig enzyme | Oryctolagus cuniculus | ? | - |
? | |
| benzenedihydrodiol + NADP+ | - |
Oryctolagus cuniculus | ? | - |
? | |
| codeine + NAD(P)+ | codeine best substrate | Oryctolagus cuniculus | codeinone + NAD(P)H | - |
? | |
| cyclohexene-2-ol + NADP+ | - |
Oryctolagus cuniculus | cyclohexene-2-one + NADPH | - |
? | |
| dihydrocodeine + NADP+ | - |
Oryctolagus cuniculus | dihydrocodeinone + NADPH | - |
? | |
| ethylmorphine + NAD(P)+ | - |
Oryctolagus cuniculus | ? | - |
? | |
| morphine + NADP+ | enzyme acitivity with NAD+ four times that with NADP+ | Oryctolagus cuniculus | morphinone + NADPH + H+ | - |
? | |
| normorphine + NAD(P)+ | - |
Oryctolagus cuniculus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 38000, SDS-PAGE | Oryctolagus cuniculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.6 | - |
with NADP+ as cofactor, in glycine-NaCl-NaOH buffer | Oryctolagus cuniculus |
| 9.4 | - |
with NAD+ as cofactor, in glycine-NaCl-NaOH buffer | Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD(P)+ | NAD+ preferred cofactor | Oryctolagus cuniculus | |
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