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Literature summary for 1.1.1.216 extracted from

  • Mayoral, J.G.; Nouzova, M.; Navare, A.; Noriega, F.G.
    NADP+-dependent farnesol dehydrogenase, a corpora allata enzyme involved in juvenile hormone synthesis (2009), Proc. Natl. Acad. Sci. USA, 106, 21091-21096.
    View publication on PubMed
Search for more literature for 1.1.1.216

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aedes aegypti

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.091
-
 (2E,6E)-farnesol pH 10.0, 30°C Aedes aegypti
0.097
-
 (2Z,6Z)-farnesol pH 10.0, 30°C Aedes aegypti
0.109
-
 (Z)-nerol pH 10.0, 30°C Aedes aegypti
0.133
-
 citronellol pH 10.0, 30°C Aedes aegypti
0.184
-
 2-decanol pH 10.0, 30°C Aedes aegypti
0.195
-
 Octanol pH 10.0, 30°C Aedes aegypti
0.208
-
 (E)-geraniol pH 10.0, 30°C Aedes aegypti

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 2 * 29000, calculated Aedes aegypti
60000
-
 gel filtration Aedes aegypti

Organism

Organism UniProt Comment Textmining
Aedes aegypti D2WKD9 NADP+-dependent farnesol dehydrogenase 1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aedes aegypti

Source Tissue

Source Tissue Comment Organism Textmining
brain and midgut, of adult female, highest levels of mRNA Aedes aegypti
-
corpora allata no farnesol dehydrogenase activity in newly emerged mosquitoes but significant activity 24 h later. SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis Aedes aegypti
-
fat body and Malpighian tubules, considerable levles of mRNA Aedes aegypti
-
ganglion abdominal and thoracic ganglia, high level of mRNA Aedes aegypti
-
malpighian tubule and fat body, considerable levles of mRNA Aedes aegypti
-
midgut and brain, of adult female, highest levels of mRNA Aedes aegypti
-
testis of adult male, substantial level of mRNA Aedes aegypti
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
9
-
 substrate (2Z,6Z)-farnesol, pH 10.0, 30°C Aedes aegypti
9.7
-
 substrate (2E,6E)-farnesol, pH 10.0, 30°C Aedes aegypti

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2E,6E)-farnesol + NADP+ enzyme shows highest affinity for farnesol Aedes aegypti (2E,6E)-farnesal + NADPH + H+
-
 ir
(2Z,6Z)-farnesol + NADP+ enzyme shows highest affinity for farnesol Aedes aegypti (2Z,6Z)-farnesal + NADPH + H+
-
 r
(E)-geraniol + NADP+
-
 Aedes aegypti (E)-geranial + NADPH + H+
-
 ?
(Z)-nerol + NADP+
-
 Aedes aegypti (Z)-neral + NADPH + H+
-
 ?
2-decanol + NADP+
-
 Aedes aegypti ? + NADPH + H+
-
 ?
citronellol + NADP+
-
 Aedes aegypti citronellal + NADPH + H+
-
 ?
additional information no activity is detected for butanol, ethanol, or glycerol, or for farnesal or farnesoic acid Aedes aegypti ?
-
 ?
octanol + NADP+
-
 Aedes aegypti octanal + NADPH + H+
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 29000, calculated Aedes aegypti

Synonyms

Synonyms Comment Organism
NADP+-dependent farnesol dehydrogenase 1
-
 Aedes aegypti
SDR-1
-
 Aedes aegypti

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10 11
-
 Aedes aegypti

Cofactor

Cofactor Comment Organism Structure
NADP+ absolutely required, NAD+ or FAD cannot substitute Aedes aegypti

Expression

Organism Comment Expression
Aedes aegypti SDR-1 mRNA levels are very low in the inactive corpora allata of the newly emerged female, but increased over 30-fold 24 h later during the peak of juvenile hormone synthesis additional information