Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | ALR shows a high level of specificity towards glutathione conjugates and very little specificity towards trypanothione conjugates | Leishmania donovani |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli cells | Leishmania donovani |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.112 | - |
methylglyoxal | recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM glutathione | Leishmania donovani | |
0.4 | - |
methylglyoxal | recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM trypanothione | Leishmania donovani |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31700 | - |
subunit, calculated from amino acid sequence | Leishmania donovani |
74000 | - |
recombinant enzyme with a maltose binding tag, SDS-PAGE | Leishmania donovani |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | - |
isolate 2001-S | - |
Purification (Comment) | Organism |
---|---|
amylose resin column chromatography | Leishmania donovani |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania donovani | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetol + NADPH | - |
Leishmania donovani | propane-1,2 diol + NADP+ | - |
? | |
methylglyoxal + NADPH + H+ | - |
Leishmania donovani | acetol + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aldose reductase | - |
Leishmania donovani |
ALR | - |
Leishmania donovani |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
44.4 | - |
methylglyoxal | recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM trypanothione | Leishmania donovani | |
78.4 | - |
methylglyoxal | recombinant enzyme, in 100 mM sodium phosphate buffer (pH 7.0), at 25°C, in the presence of 0.3 mM glutathione | Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Leishmania donovani |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leishmania donovani | calculated from amino acid sequence | - |
5.85 |